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Series GSE55108 Query DataSets for GSE55108
Status Public on May 14, 2015
Title Loss of immunosuppressive and anti-inflammatory properties in transformed human mesenchymal stem cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We used collection of sequentially mutated BM-hMSCs ranging from wild type (no oncogenic hits) to fully transformed hMSCs (targeted with up six oncogenic mutations)to address whether BM-hMSCs at different stages of a well-characterized oncogenic process (normal, immortalized, transformed) retain immunomodulatory properties in vitro and in vivo. We characterize, for the first time, an oncogenic transformation-associated loss of the immunesuppressive and anti-inflammatory properties by hMSCs and identify candidate immune effectors underlying the loss of immunomodulation in transformed hMSCs.
Overall design Wild type BM-hMSCs (MSC-0H-GFP) did not develop tumors, meanwhile hMSCs transformed sequentially transformed with 5 oncogenic lesions (p53 inactivation; Rb inactivation; expression of hTERT; stabilization of c-myc; and expression of oncogenic H-RAS) (MSC-5H-GFP) cells gave rise to undifferentiated sarcomas when inoculated into immunedeficient mice. The tumoral transformation process causes the loss of the immunosuppressive and anti-inflamatory propierties of MSC-5H-GFP cells as compared to those of the wild type MSC-0H-GFP cells. To understand the basis of this loss of immunosuppression in transformed hMSCs, gene expression analysis was performed to create the list of differentially expressed genes between MSC-0H-GFP and MSC-5H-GFP cells. Further analysis of altered signaling pathways and upstream regulators were performed using the Ingenuity Pathway Analysis software.
Contributor(s) Rodriguez R, Menendez P, Delgado M, Araúzo-Bravo MJ
Citation(s) 25358789
Submission date Feb 18, 2014
Last update date Jan 15, 2022
Contact name Marcos J. Araúzo-Bravo
Phone +34 943 00 6108
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bionformatics
Street address Rogentstrasse
City Muenster
ZIP/Postal code 48149
Country Germany
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (4)
GSM1329710 MSC-0H-GFP_rep1
GSM1329711 MSC-0H-GFP_rep2
GSM1329712 MSC-5H-GFP_rep1
BioProject PRJNA238542

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE55108_RAW.tar 18.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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