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Series GSE54942 Query DataSets for GSE54942
Status Public on Feb 27, 2014
Title Identification of target genes regulated by ΔNp63α in SaOS-2 cells stably expressed with ΔNp63α
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To identify the target gene of ΔNp63α, in osteosarcoma cells, we performed the global gene expression profiling in SaOS- 2 cells stably overexpressing the ΔNp63α gene (SaOS- 2-ΔNp63α). ΔNp63α target genes were identified by comparison with genes expressed from SaOS-2-EV transfected with an empty vector ( SaOS-2-EV).
Overall design Total RNA was extracted from SaOS- 2-ΔNp63α and SaOS-2-EV cells cultured in three independent replicates for each condition. Cy3-labeled cRNA was synthesized and hybridized to Agilent human 8 x 60K microarrays. Raw data, obtained after array image analysis were normalized and used to retrieve differentially expressed genes in ΔNp63α overexpressing SaOS- 2 cells and genes from control SaOS-2-EV cells.
Contributor(s) Ram Kumar RM, Walter B, Lecca MR, Fuchs B
Citation(s) 25085524
Submission date Feb 12, 2014
Last update date Nov 27, 2018
Contact name Ram Mohan Ram Kumar
Organization name University Hospital Balgrist, University of Zurich, Zurich, Switzerland
Department Orthopaedics
Street address Forchstrasse 340
City Zurich
ZIP/Postal code 8008
Country Switzerland
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (6)
GSM1326870 EV_1
GSM1326871 EV_2
GSM1326872 EV_3
BioProject PRJNA239403

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE54942_RAW.tar 23.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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