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Status |
Public on Feb 27, 2014 |
Title |
Identification of target genes regulated by ΔNp63α in SaOS-2 cells stably expressed with ΔNp63α |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
To identify the target gene of ΔNp63α, in osteosarcoma cells, we performed the global gene expression profiling in SaOS- 2 cells stably overexpressing the ΔNp63α gene (SaOS- 2-ΔNp63α). ΔNp63α target genes were identified by comparison with genes expressed from SaOS-2-EV transfected with an empty vector ( SaOS-2-EV).
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Overall design |
Total RNA was extracted from SaOS- 2-ΔNp63α and SaOS-2-EV cells cultured in three independent replicates for each condition. Cy3-labeled cRNA was synthesized and hybridized to Agilent human 8 x 60K microarrays. Raw data, obtained after array image analysis were normalized and used to retrieve differentially expressed genes in ΔNp63α overexpressing SaOS- 2 cells and genes from control SaOS-2-EV cells.
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Contributor(s) |
Ram Kumar RM, Walter B, Lecca MR, Fuchs B |
Citation(s) |
25085524 |
Submission date |
Feb 12, 2014 |
Last update date |
Nov 27, 2018 |
Contact name |
Ram Mohan Ram Kumar |
E-mail(s) |
rkumar@research.balgrist.ch
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Organization name |
University Hospital Balgrist, University of Zurich, Zurich, Switzerland
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Department |
Orthopaedics
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Street address |
Forchstrasse 340
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City |
Zurich |
ZIP/Postal code |
8008 |
Country |
Switzerland |
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Platforms (1) |
GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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Samples (6)
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Relations |
BioProject |
PRJNA239403 |