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| Status |
Public on Oct 14, 2014 |
| Title |
Comparison of gene expression pattern between Wild-type and Setd2 knockout mESCs [Affymetrix] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
SETD2/HYPB has been known as a histone H3K36 specific methyltransferase. However, its roles in physiology such as development and cellular function remain unclear. In this study, using mESCs as cellular model, we show that Setd2 mainly regulates differentiation of murine embryonic stem cells (mESCs) towards primitive endoderm. This study aimed at exploring how did Setd2 regulate primitive endoderm. differentiation.
We used microarrays to detail the global programme of gene expression controled by setd2, which is required for endoderm differentiation.
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| Overall design |
Wild type and Setd2 knockout mESCs were selected for RNA extraction and hybridization on Affymetrix GeneChip® mouse genome 430 2.0 arrays. We sought to obtain some deregulated genes, which were required for primitive endoderm differentiation. For comparison, three biological repeats of each were performed.
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| Contributor(s) |
Zhang Y, Chen Z, Huang Q, Chen S |
| Citation(s) |
25242323 |
| Submission date |
Feb 12, 2014 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Qiuhua Huang |
| E-mail(s) |
qiuhua_huang@126.com
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| Phone |
0086-21-34187717
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| Organization name |
Shanghai Institute of Hematology
|
| Street address |
197 ruijin road II
|
| City |
Shanghai |
| ZIP/Postal code |
200025 |
| Country |
China |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE54933 |
Gene expression pattern between Wild-type and Setd2 knockout mESCs |
|
| Relations |
| BioProject |
PRJNA238053 |
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