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Series GSE54931 Query DataSets for GSE54931
Status Public on Oct 14, 2014
Title Comparison of gene expression pattern between Wild-type and Setd2 knockout mESCs [Affymetrix]
Organism Mus musculus
Experiment type Expression profiling by array
Summary SETD2/HYPB has been known as a histone H3K36 specific methyltransferase. However, its roles in physiology such as development and cellular function remain unclear. In this study, using mESCs as cellular model, we show that Setd2 mainly regulates differentiation of murine embryonic stem cells (mESCs) towards primitive endoderm. This study aimed at exploring how did Setd2 regulate primitive endoderm. differentiation.
We used microarrays to detail the global programme of gene expression controled by setd2, which is required for endoderm differentiation.
Overall design Wild type and Setd2 knockout mESCs were selected for RNA extraction and hybridization on Affymetrix GeneChip® mouse genome 430 2.0 arrays. We sought to obtain some deregulated genes, which were required for primitive endoderm differentiation. For comparison, three biological repeats of each were performed.
Contributor(s) Zhang Y, Chen Z, Huang Q, Chen S
Citation(s) 25242323
Submission date Feb 12, 2014
Last update date Feb 11, 2019
Contact name Qiuhua Huang
Phone 0086-21-34187717
Organization name Shanghai Institute of Hematology
Street address 197 ruijin road II
City Shanghai
ZIP/Postal code 200025
Country China
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM1326660 Setd2 wild type mESCs, biological rep1
GSM1326661 Setd2 wild type mESCs, biological rep2
GSM1326662 Setd2 wild type mESCs, biological rep3
This SubSeries is part of SuperSeries:
GSE54933 Gene expression pattern between Wild-type and Setd2 knockout mESCs
BioProject PRJNA238053

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE54931_RAW.tar 19.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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