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Series GSE54845 Query DataSets for GSE54845
Status Public on Sep 15, 2014
Title MacroH2A1 ChIP-seq from IMR90 human primary lung fibroblasts
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary The histone variant macroH2A1 and the poly(ADP-ribose) polymerase PARP-1 both regulate gene transcription by modulating chromatin structure and function. Of the two macroH2A1 splice variants, macroH2A1.1 and macroH2A1.2, the former is often suppressed in cancer and has the unique ability to interact with poly(ADP-ribose). Using ChIP-seq in primary lung fibroblasts, we demonstrate that macroH2A1 is incorporated into either of two spatially and functionally distinct types of chromatin; the first is marked by H3 K27 trimethylation, while the second contains a set of nine histone acetylations. MacroH2A1-regulated genes are involved in cancer progression are specifically found in macroH2A1-containing acetylated chromatin. Through the recruitment of PARP-1, macroH2A1.1 promotes the acetylation of H2B K12 and K120 which plays a key role in the regulation of macroH2A1 target genes in primary cells. The macroH2A1/PARP-1 pathway regulating H2B K12 and K120 acetylation is disrupted in cancer cells, in part, explaining macroH2A1’s role in cancer suppression.
Overall design Two biological replicates of the macroH2A1 ChIP and two corresponding input samples were sequenced
Contributor(s) Gamble MJ, Chen H
Citation(s) 25306110
Submission date Feb 10, 2014
Last update date May 15, 2019
Contact name Matthew Jon Gamble
Organization name Albert Einstein College of Medicine
Department Molecular Pharmacology
Lab Gamble Lab
Street address 1300 Morris Park Ave / Golding 203
City Bronx
State/province NY
ZIP/Postal code 10461
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (4)
GSM1325070 macroH2A1 ChIP replicate 1
GSM1325071 input replicate 1
GSM1325072 macroH2A1 ChIP replicate 2
This SubSeries is part of SuperSeries:
GSE54847 Exploring the role of macroH2A1 in transcription regulation in IMR90 primary human lung fibroblasts with RNA-seq and ChIP-seq
BioProject PRJNA237811
SRA SRP037549

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Supplementary file Size Download File type/resource
GSE54845_RAW.tar 3.4 Mb (http)(custom) TAR (of BEDGRAPH, GAPPEDPEAK)
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Raw data are available in SRA
Processed data provided as supplementary file

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