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Series GSE54841 Query DataSets for GSE54841
Status Public on Oct 20, 2014
Title Acute depletion redefines the division of labor among DNA methyltransferases in methylating the human genome [gene expression]
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Global patterns of DNA methylation, mediated by the DNA methyltransferases (DNMTs), are disrupted in all cancers by mechanisms that remain largely unknown, hampering their development as therapeutic targets. Combinatorial acute depltion of all DNMTs in a pluripotent human tumor cell line, followed by epigenome and transcriptome analysis, revealed DNMT functions in unprecedented detail. DNMT3B occupancy regulates methylation during differentiation, while an unexpected interplay was discovered in which DNMT1 and DNMT3B antithetically regulate methylation and hydroxymethylation in gene bodies, a finding confirmed in other cell types. DNMT3B mediated nonCpG methylation, while DNMT3L influenced the activity of DNMT3B toward nonCpG versus CpG site methylation. Taken together, these data reveal new functional targets of each DNMT suggesting that isoform selective inhibition would be therapeutically advantageous.
Overall design Affymetrix gene expression Human Gene 1.0 ST microarray of NCCIT human embryonic carcinoma cells (13 samples in duplicate).
Contributor(s) Tiedemann RL, Putiri EL, Lee JH, Hlady RA, Kashiwagi K, Ordog T, Zhang Z, Liu C, Choi JH, Robertson KD
Citation(s) 25453758
Submission date Feb 10, 2014
Last update date Jul 26, 2018
Contact name Keith D Robertson
Phone 507-266-4886
Organization name Mayo Clinic
Department Molecular Pharmacology & Experimental Therapeutics
Lab Epigenetic Etiology of Human Disease Laboratory
Street address 200 First Street SW, Stabile 12-70
City Rochester
State/province MN
ZIP/Postal code 55905
Country USA
Platforms (1)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Samples (26)
GSM1325009 NCCIT siNTC-1 rep1
GSM1325010 NCCIT siNTC-1 rep2
GSM1325011 NCCIT siNTC-2 rep1
This SubSeries is part of SuperSeries:
GSE54843 Acute depletion redefines the division of labor among DNA methyltransferases in methylating the human genome
BioProject PRJNA237804

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE54841_RAW.tar 111.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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