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Series GSE54791 Query DataSets for GSE54791
Status Public on Apr 14, 2014
Title Reconstructing and reprogramming the tumor propagating potential of glioblastoma stem-like cells: RNA-seq
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Developmental fate decisions are dictated by master transcription factors (TFs) that interact with cis-regulatory elements to direct transcriptional programs. Certain malignant tumors may also depend on a cellular hierarchy reminiscent of normal development but superimposed on underlying genetic aberrations. In glioblastoma (GBM), a subset of stem-like tumor- propagating cells (TPCs) appears to drive tumor progression and underlie therapeutic resistance, yet remain poorly understood. Here, we identify a core set of neurodevelopmental TFs (POU3F2, SOX2, SALL2, OLIG2) essential for GBM propagation. These TFs coordinately bind and activate TPC-specific regulatory elements, and are sufficient to fully reprogram differentiated GBM cells to ‘induced’ TPCs that recapitulate the epigenetic landscape and phenotype of native TPCs. We reconstruct a TF network model that highlights critical interactions and identifies novel therapeutic targets for eliminating TPCs. Our study establishes the epigenetic basis of a developmental hierarchy in a devastating malignancy, provides detailed insight into the underlying gene regulatory programs, and suggests attendant therapeutic strategies.
Overall design 3'end RNA sequencing

Submitter declares that the raw data are being deposited in dbGaP.
Contributor(s) Suvà ML, Rheinbay E, Gillespie SM, Bernstein BE
Citation(s) 24726434
Submission date Feb 07, 2014
Last update date Jun 07, 2020
Contact name Esther Rheinbay
Organization name Broad Institute
Street address 7 Cambridge Center
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (23)
GSM1324075 MGG4TPC_1
GSM1324076 MGG4TPC_2
GSM1324077 MGG4TPC_3
This SubSeries is part of SuperSeries:
GSE54792 Epigenomic profiling of stemness, differentiation and primary tissues in human glioblastoma
BioProject PRJNA237655

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE54791_RAW.tar 282.9 Mb (http)(custom) TAR (of BW)
GSE54791_TPC_DGC_CPM.txt.gz 1.1 Mb (ftp)(http) TXT
GSE54791_iTPC_empty_CPM.txt.gz 272.0 Kb (ftp)(http) TXT
Raw data not provided for this record
Processed data provided as supplementary file
Processed data are available on Series record

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