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| Status |
Public on Jun 03, 2015 |
| Title |
Gene expression in near isogenic spring wheat lines carrying or not the 2DL FHB resistance QTL during early infection with Fusarium graminearum, the major causal agent of fusarium head blight in wheat |
| Organism |
Triticum aestivum |
| Experiment type |
Expression profiling by array
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| Summary |
Fusarium head blight (FHB) is a major disease of cereal crops caused by the fungus Fusarium graminearum (Fg). FHB affects the flowering heads (or spikes). This study compare the gene expression profile in wheat spikelets from near isogenic spring wheat lines carrying or not the FHB resistance QTL 2DL, after inoculation with water (H2O) or Fg; two inoculation methods were also compared, point and spray inoculation.
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| Overall design |
We used the Affymetrix wheat array to compare wheat spikelets from the near isogenic spring wheat lines 2-2890 (nul for FHB resistance QTLs 2DL, 3BS and 5A), 2-2618 (carrying 2DL QTL; nul for QTLs 3BS, 5A; sister line to 2-2890) and 2-2674 (nul for QTLs 2DL, 3BS and 5A), after inoculation with water (H2O) or Fg. Two inoculation methods were also compared, point and spray inoculation. Samplings were done at 4 days (d) after inoculation, for 2 (point inoculation) or 3 (spray inoculation) biological replicates
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| Contributor(s) |
Ouellet T, Gulden S |
| Citation(s) |
25903423 |
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| Submission date |
Jan 30, 2014 |
| Last update date |
Dec 04, 2018 |
| Contact name |
Thérèse Ouellet |
| E-mail(s) |
therese.ouellet@canada.ca
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| Phone |
613-759-1658
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| Organization name |
Agriculture and Agri-Food Canada
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| Department |
Eastern Cereal and Oilseed Research Centre
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| Street address |
960 Carling Ave, room 2091
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| City |
Ottawa |
| State/province |
ON |
| ZIP/Postal code |
K1A 0C6 |
| Country |
Canada |
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| Platforms (1) |
| GPL3802 |
[wheat] Affymetrix Wheat Genome Array |
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| Samples (30)
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| GSM1318687 |
2-2618, 4 d after water inoculation by point, rep 1 |
| GSM1318688 |
2-2618, 4 d after water inoculation by point, rep 2 |
| GSM1318689 |
2-2618, 4 d after Fg inoculation by point, rep 2 |
| GSM1318690 |
2-2618, 4 d after Fg inoculation by point, rep 3 |
| GSM1318691 |
2-2890, 4 d after water inoculation by point, rep 1 |
| GSM1318692 |
2-2890, 4 d after water inoculation by point, rep 2 |
| GSM1318693 |
2-2890, 4 d after Fg inoculation by point, rep 2 |
| GSM1318694 |
2-2890, 4 d after Fg inoculation by point, rep 3 |
| GSM1318695 |
2-2674, 4 d after water inoculation by point, rep 1 |
| GSM1318696 |
2-2674, 4 d after water inoculation by point, rep 2 |
| GSM1318697 |
2-2674, 4 d after Fg inoculation by point, rep 2 |
| GSM1318698 |
2-2674, 4 d after Fg inoculation by point, rep 3 |
| GSM1318699 |
2-2618, 4 d after water inoculation by spray, rep 1 |
| GSM1318700 |
2-2618, 4 d after water inoculation by spray, rep 2 |
| GSM1318701 |
2-2618, 4 d after water inoculation by spray, rep 3 |
| GSM1318702 |
2-2618, 4 d after Fg inoculation by spray, rep 1 |
| GSM1318703 |
2-2618, 4 d after Fg inoculation by spray, rep 2 |
| GSM1318704 |
2-2618, 4 d after Fg inoculation by spray, rep 3 |
| GSM1318705 |
2-2890, 4 d after water inoculation by spray, rep 2 |
| GSM1318706 |
2-2890, 4 d after water inoculation by spray, rep 3 |
| GSM1318707 |
2-2890, 4 d after water inoculation by spray, rep 4 |
| GSM1318708 |
2-2890, 4 d after Fg inoculation by spray, rep 1 |
| GSM1318709 |
2-2890, 4 d after Fg inoculation by spray, rep 2 |
| GSM1318710 |
2-2890, 4 d after Fg inoculation by spray, rep 3 |
| GSM1318711 |
2-2674, 4 d after water inoculation by spray, rep 1 |
| GSM1318712 |
2-2674, 4 d after water inoculation by spray, rep 2 |
| GSM1318713 |
2-2674, 4 d after water inoculation by spray, rep 3 |
| GSM1318714 |
2-2674, 4 d after Fg inoculation by spray, rep 1 |
| GSM1318715 |
2-2674, 4 d after Fg inoculation by spray, rep 2 |
| GSM1318716 |
2-2674, 4 d after Fg inoculation by spray, rep 3 |
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| This SubSeries is part of SuperSeries: |
| GSE54556 |
Gene expression during early infection by Fusarium graminearum in wheat lines susceptible and resistant to fusarium head blight |
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| Relations |
| BioProject |
PRJNA237029 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE54550_RAW.tar |
237.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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