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Series GSE54462 Query DataSets for GSE54462
Status Public on Apr 07, 2015
Title Cell type-specific expression profile and signaling requirements in early hematopoietic reprogramming
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Hematopoietic cells represent an attractive starting cell type for induced pluripotent stem (iPS) cells induction, yet the molecular mechanisms in hematopoietic reprogramming are poorly defined. In this study, we showed that long-term hematopoietic stem cells are more amenable for iPS cells induction among several hematopoietic stem and progenitor cell (HSPC) populations, and that this is accompanied by an earlier induction of the transcriptional program that is involved in the promotion of macromolecule metabolism and cell proliferation. Notably, we identified multiple signaling pathways that exhibited distinct expression patterns in HSPCs compared to that of fibroblasts, which is the most commonly used model for probing the somatic reprogramming process. We further experimentally confirmed the differential requirements of the Wnt/β-catenin and transforming growth factor-beta (TGF-β) signaling pathways in these two cell types. These data demonstrate that hematopoietic cells have a cell-type specific transcriptional program and possess unique signaling requirements in the early phase of reprogramming.
Overall design Examine the differential global gene expression among different hematopietic cells in reprogramming. LT-HSC, ST-HSC, MP and fibroblasts were used in this study. Doxcycline addition could induce expression of oct4, sox2, klf4 and c-myc, consequently reprogramming these cells into iPS cells. Hematopietic cells cultured with doxcycline for 0, 2, 4 days were sampled, and samples at the same timepoint without doxcycline were also taken to exclude vast gene expression change in hematopoietic cell in-vitro culture. Fibroblast samples induced by doxcycline for 0, 2, 4 days were also introduced to provide comparism between lineages.
Contributor(s) Kang L, Wu J, Tao Y, Liu X, Ma Y, Gao S, Wang Q
Citation(s) 25751301
Submission date Jan 28, 2014
Last update date May 15, 2019
Contact name Lan Kang
Organization name Dalian Medical University
Department Institute of Cancer Stem Cell
Street address 9 West Section, Lvshun South Road
City Dalian
ZIP/Postal code 116044
Country China
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (18)
GSM1315828 LT-HSC_0
GSM1315829 ST-HSC_0
GSM1315830 MP_0
BioProject PRJNA236573
SRA SRP036027

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GSE54462_Processed_data_file.txt.gz 1.1 Mb (ftp)(http) TXT
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