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Series GSE54449 Query DataSets for GSE54449
Status Public on Aug 08, 2016
Title Negative reciprocal regulation between SIRT1 and Per2 in circadian clock during aging (ChIP-Seq)
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary SIRT1 is involved in both aging and circadian clock regulation, yet the link between the two processes in relation to SIRT1 function is unclear. Analyzing SIRT1-deficient cells and mice, we demonstrated that SIRT1 and Per2 constitute a reciprocal negative regulation loop that plays important roles in modulating circadian rhythmicity, metabolism and aging. SIRT1-deficient mice exhibit profound premature aging and enhanced H4K16 acetylation in the promoter of Per2 leading to its overexpression; in turn, Per2 suppresses SIRT1 transcription through binding to SIRT1 promoter at the CLOCK/BMAL1 site. We further demonstrated that absence of SIRT1 or ectopic overexpression of Per2 in the liver resulted in an accelerated pace of circadian rhythm and dysregulated amplitude, mimicking the natural process of circadian shortening in aged mice. Thus the interplay between SIRT1 and Per2 provides a link between the life-long sequence of aging and circadian clock maintenance.
Overall design To test whether changes in gene expression induced by Sirt1 KO are associated with changes in H4K16ac level at promoters, we profiled gene expression by using microarray and genomic binding of H4K16ac by using ChIP-Seq for Sirt1 KO mice and wild type control month (3-month).
Contributor(s) Rui-Hong W, Tingrui Z, Kairong C, Gangqing H, Qiang C, Weiping C, Keji Z, Chu-Xia D
Citation(s) 27346580
Submission date Jan 28, 2014
Last update date May 15, 2019
Contact name Gangqing Hu
Organization name West Virginia University
Department MicroBiology, Immunology, and Cell Biology
Lab 2072A, HSC North, Floor 2
Street address 64 Medical Center Drive
City Morgantown
State/province West Virginia
ZIP/Postal code 26506-9177
Country USA
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (4)
GSM1315584 GA1838-mouse-9732-Input
GSM1315585 GA1839-mouse-9905-Input
GSM1315586 GA1842-mouse-9732-H4K16ac
This SubSeries is part of SuperSeries:
GSE54451 Negative reciprocal regulation between SIRT1 and Per2 in circadian clock during aging
BioProject PRJNA236564
SRA SRP035965

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Supplementary file Size Download File type/resource
GSE54449_GA1842_mouse_9732_H4K16ac_noDup-W200-G400-islands-summary-FDR0.001.txt.gz 833.4 Kb (ftp)(http) TXT
GSE54449_GA1843_mouse_9905_H4K16ac_noDup-W200-G400-islands-summary-FDR0.001.txt.gz 735.4 Kb (ftp)(http) TXT
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