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Series GSE54344 Query DataSets for GSE54344
Status Public on Mar 01, 2015
Title Aberrant chromatin acetylation in MLL-AF9 leukemia mediates the response to HDAC inhibition (ChIP-Seq)
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We studied the chromatin modification patterns induced by the presence of the MLL-AF9 fusion protein in a model of human hematopoietic stem/progenitor cells (HSPC) transduced with retrovirus expressing MLL-AF9cDNA (HSPC-MA9). Comparative ChIP-seq analysis between HSPC-MA9 and control HSPC, revealed a massive hyperacetylation of histones that was consistent with the transcriptional profile in the presence of MLL-AF9 fusion protein. Furthermore, we identified 66 MLL-AF9 targets, and found that H4ac was present along with H3K4me3 and H3K79me2 chromatin marks in over 50% of the MLL-AF9 target genes.
Overall design Examination of histone aceylation and methylation changes upon expression of MLL-AF9 fusion protein in human hematopoietic stem/progenitor cells.
Contributor(s) Maiques-Diaz A, Eguileor A, Shrestha M, Gómez López G, Wunderlich M, del Rio-Machin A, Santoyo-Lopez J, Agirre X, Mulloy JC, Cigudosa JC, Alvarez S
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Submission date Jan 23, 2014
Last update date May 15, 2019
Contact name Sara Alvarez
Phone +34 672 05 98 89
Organization name NIMGenetics
Street address C/Faraday 7
City Madrid
State/province Madrid
ZIP/Postal code 28049
Country Spain
Platforms (1)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
Samples (9)
GSM1313522 MLL_HSPC-MA9cells_ChIPseq
GSM1313523 AF9_HSPC-MA9cells_ChIPseq
GSM1313524 H3K4me3_HSPC-MA9cells_ChIPseq
This SubSeries is part of SuperSeries:
GSE54348 Aberrant chromatin acetylation in MLL-AF9 leukemia mediates the response to HDAC inhibition
BioProject PRJNA236241
SRA SRP035623

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Supplementary file Size Download File type/resource
GSE54344_RAW.tar 28.2 Mb (http)(custom) TAR (of BED, WIG)
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Raw data are available in SRA
Processed data provided as supplementary file

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