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Series GSE54231 Query DataSets for GSE54231
Status Public on Jun 01, 2014
Title Histone H3 Acetylation and microRNA(s) Regulate Inflammatory response in Mastitis Mice, induced by Staphylococcus aureus Infection [smallRNA-Seq]
Organism Mus musculus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Bacterial infection in the mammary gland parenchyma induces local inflammation that can lead to a multietiological complex disease called mastitis. Globally Staphylococcus aureus is the single largest mastitis pathogen and the infection can ultimately result in either subclinical or chronic and sometimes lifelong infection. In the present report we have addressed the differential inflammatory response in the mice mammary tissue during intramammary infection and the altered epigenetic context induced by two closely related strains of S. aureus. Immunohistochemical and immunoblot analysis showed strain specific hyperacetylation at histone H3K9 and H3K14 residues. Real-time PCR and genome-wide gene expression studied showed expression of a set of proinflammatory genes and cytokines in a temporal manner. Remarkably, over expression of the genes significantly correlated with the promoter specific acetylation in these residues. Furthermore, we have identified several differentially expressed known miRNAs and 4 novel miRNAs in the S. aureus infected mice mammary tissue by small RNA sequencing. By employing these gene expression data, an attempt has been made to delineate the gene regulatory networks in the strain specific inflammatory response. Apparently, one of the isolates of S. aureus activated the NFkB signaling leading to drastic inflammatory response and induction of immune surveillance, which could lead to rapid clearance of the pathogen. The other strain repressed most of the inflammatory response, which might help in its sustenance in the host tissue. Taken together, our studies shed substantial lights to understand the mechanisms of strain specific differential inflammatory response to S. aureus infection during mastitis.
Overall design One control and two samples infected with two different strains of S. aureus
Contributor(s) Modak R, Das Mitra S, Shome BR, Kundu TK
Citation(s) 25075227
Submission date Jan 21, 2014
Last update date May 15, 2019
Contact name Tapas Kumar Kundu
Phone +91-80-22082840
Organization name Jawaharlal Nehru Centre for Advanced Scientific Research
Department Molecular Biology & Genetics Unit
Lab Transcription & Disease Laboratory
Street address Jakkur P.O.
City Bangalore
State/province Karnataka
ZIP/Postal code 560064
Country India
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (3)
GSM1310519 Mice_mammary_PBS_smallRNA
GSM1310520 Mice_mammary_SA1_smallRNA
GSM1310521 Mice_mammary_SA2_smallRNA
This SubSeries is part of SuperSeries:
GSE54232 Histone H3 Acetylation and microRNA(s) Regulate Inflammatory response in Mastitis Mice, induced by Staphylococcus aureus Infection
BioProject PRJNA235998
SRA SRP035553

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Supplementary file Size Download File type/resource
GSE54231_RPKM_DATA.txt.gz 116.8 Kb (ftp)(http) TXT
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Processed data are available on Series record

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