GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE54122 Query DataSets for GSE54122
Status Public on Jan 17, 2014
Title Next Generation Sequencing of MCF-7 and MDA-MB-435 cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Human breast cancer cells MDA-MB-435 had higher metastatic potential and other aggressive characteristics than MCF-7 cells. Next-generation RNA sequencing (RNA-Seq) was used to clear this concern through comparison the transcriptomic expression profiles of these two cells.
Overall design Total RNA were extracted from MCF-7 and MDA-MB-435S cells, and the polyA+ mRNA was sequenced using Illumina Genome Analyzer IIx. The reads were mapped to RefSeq RNA reference sequences.
Contributor(s) Gao X, Wang C
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jan 15, 2014
Last update date May 15, 2019
Contact name Cuihua Wang
Organization name Guangzhou Lab
Street address 601 Huangpu Ave, Guangzhou
City Guangzhou
State/province Guangdong
ZIP/Postal code 510632
Country China
Platforms (1)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
Samples (2)
GSM1308330 MCF-7mRNA
GSM1308331 MDA-MB-435SmRNA
BioProject PRJNA235273
SRA SRP035461

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE54122_RAW.tar 710.0 Kb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap