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Series GSE54114 Query DataSets for GSE54114
Status Public on Feb 28, 2014
Title Poly(A) tail length change of miRNA targets in HeLa cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary MicroRNA induces deadenylation of its targets according to the current model in the scientific community, but this model is based on the studies of a few individual genes. We tested the model by examining the global effect of miRNA on poly(A) tail of the targets. Synthetic miR-1 mimic was transfected into HeLa cells, and the poly(A) length was measured by TAIL-seq. Deadenylation of miR-1 targets was evident as early as 3 hours post-transfection without a significant change in mRNA level. After 6 or 9 hours, target mRNA level was substantially downregulated. Therefore, although there are some exceptions, our result confirms that, in general, miRNA indeed induces deadenylation. Furthermore, our kinetic global analysis confirms that deadenylation precedes mRNA decay.
Overall design Four culture dishes of HeLa cells were treated with miR-1 all together, then collected and prepared for sequencing after different time of incubation (0, 3, 6, and 9 hours post-transfection)
Contributor(s) Ha M, Chang H, Kim VN
Citation(s) 24582499
Submission date Jan 15, 2014
Last update date May 15, 2019
Contact name Hyeshik Chang
Organization name Seoul National University
Department School of Biological Sciences
Lab Hyeshik Chang Lab
Street address Building 203 Room 525, School of Biological Sciences, Seoul National University, 1 Gwanak-ro, Gwanak-gu
City Seoul
State/province South Korea
ZIP/Postal code 08826
Country South Korea
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (4)
GSM1308257 HeLa-miR-1-0h
GSM1308258 HeLa-miR-1-3h
GSM1308259 HeLa-miR-1-6h
BioProject PRJNA235199
SRA SRP035419

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Supplementary file Size Download File type/resource
GSE54114_RAW.tar 3.3 Mb (http)(custom) TAR (of CSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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