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Series GSE54089 Query DataSets for GSE54089
Status Public on May 01, 2014
Title Testis expression in Mus musculus domesticus WSB/EiJ x Mus musculus musculus PWD/PhJ F2 hybrids
Organism Mus musculus
Experiment type Expression profiling by array
Summary To characterize the genetic basis of hybrid male sterility in detail, we used a systems genetics approach, integrating mapping of gene expression traits with sterility phenotypes and QTL. We measured genome-wide testis expression in 305 male F2s from a cross between wild-derived inbred strains of M. musculus musculus and M. m. domesticus. We identified several thousand cis- and trans-acting QTL contributing to expression variation (eQTL). Many trans eQTL cluster into eleven ‘hotspots,’ seven of which co-localize with QTL for sterility phenotypes identified in the cross. The number and clustering of trans eQTL - but not cis eQTL - were substantially lower when mapping was restricted to a ‘fertile’ subset of mice, providing evidence that trans eQTL hotspots are related to sterility. Functional annotation of transcripts with eQTL provides insights into the biological processes disrupted by sterility loci and guides prioritization of candidate genes. Using a conditional mapping approach, we identified eQTL dependent on interactions between loci, revealing a complex system of epistasis. Our results illuminate established patterns, including the role of the X chromosome in hybrid sterility.
Overall design Gene expression was measured in whole testis in males aged 70(±5) days. Samples include 294 WSB/EiJ x PWD/PhJ F2s, 11 PWD/PhJ x WSB/EiJ F2s, 8 WSB/EiJ, 8 PWD/PhJ, 6 PWD/PhJ x WSB/EiJ F1s and 4 WSB/EiJ x PWD/PhJ F1s.
Contributor(s) Turner LM
Citation(s) 24586194
Submission date Jan 15, 2014
Last update date Jan 12, 2017
Contact name Leslie M Turner
Organization name Max Planck Institute for Evolutionary Biology
Department Evolutionary Genetics
Street address August-Thienemann Str 2
City Ploen
ZIP/Postal code 24306
Country Germany
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (331)
GSM1307519 Testis_F1PW_ind1204
GSM1307520 Testis_F1PW_ind1205
GSM1307521 Testis_F1PW_ind1206
BioProject PRJNA235166

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE54089_RAW.tar 3.0 Gb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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