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Series GSE53927 Query DataSets for GSE53927
Status Public on Mar 12, 2014
Title Identification of beta-catenin binding regions in SW480 cells
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Deregulation of canonical Wnt/beta-catenin pathway is one of the earliest events in the pathogenesis of colon cancer. Mutations in APC or CTNNB1 (beta-catenin gene) are highly frequent in colon cancer and cause aberrant stabilization of b-catenin, which activates the transcription of Wnt target genes by binding to chromatin via the TCF/LEF transcription factors. Here we report an integrative analysis of genome-wide chromatin occupancy of b-catenin by chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq) and gene expression profiling by microarray analysis upon RNAi-mediated knockdown of beta-catenin in colon cancer cells (GSE53656).
Overall design Immunoprecipitated samples from human colon cancer SW480 cells with antibodies against beta-catenin and control IgG respectively were used for ChIP-seq experiments.
Contributor(s) Watanabe K, Dai X
Citation(s) 24651522
Submission date Jan 08, 2014
Last update date May 15, 2019
Contact name Kazuhide Watanabe
Phone +81-045-503-9222
Organization name Riken
Department Center for Integrative Medical Sciences
Street address 1-7-22 Suehiro-cho Tsurumi-ku
City Yokohama
State/province Kanagawa
ZIP/Postal code 230-0045
Country Japan
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (2)
GSM1303695 beta-catenin ChIP
GSM1303696 IgG Control
BioProject PRJNA233973
SRA SRP035249

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Supplementary file Size Download File type/resource
GSE53927_BetaCateninPeaks_control_afterfiting_all.wig.gz 140.6 Mb (ftp)(http) WIG
GSE53927_BetaCateninPeaks_peaks.bed.gz 146.5 Kb (ftp)(http) BED
GSE53927_BetaCateninPeaks_treat_afterfiting_all.wig.gz 143.8 Mb (ftp)(http) WIG
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