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Series GSE53919 Query DataSets for GSE53919
Status Public on Dec 17, 2014
Title RNF17 blocks promiscuous activity of PIWI proteins in mouse testes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Other
Summary PIWI proteins and their associated piRNAs protect germ cells from the activity of mobile genetic elements. Two classes of piRNAs—primary and secondary—are defined by their mechanisms of biogenesis. Primary piRNAs are processed directly from transcripts of piRNA cluster loci, whereas secondary piRNAs are generated in an adaptive amplification loop, termed the ping-pong cycle. In mammals, piRNA populations are dynamic, shifting as male germ cells develop. Embryonic piRNAs consist of both primary and secondary species and are mainly directed toward transposons. In meiotic cells, the piRNA population is transposon-poor and largely restricted to primary piRNAs derived from pachytene piRNA clusters. The transition from the embryonic to the adult piRNA pathway is not well understood. Here we show that RNF17 shapes adult meiotic piRNA content by suppressing the production of secondary piRNAs. In the absence of RNF17, ping-pong occurs inappropriately in meiotic cells. Ping-pong initiates piRNA responses against not only transposons but also protein-coding genes and long noncoding RNAs, including genes essential for germ cell development. Thus, the sterility of Rnf17 mutants may be a manifestation of a small RNA-based autoimmune reaction.
 
Overall design Refer to individual Series
 
Citation(s) 26115953
Submission date Jan 08, 2014
Last update date May 15, 2019
Contact name Vasily Vagin
E-mail(s) vagin@cshl.edu
Organization name Cold Spring Harbor
Lab Hannon's
Street address 1 Bungtown Rd
City Cold Spring Harbor
State/province NY
ZIP/Postal code 11724
Country USA
 
Platforms (3)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (38)
GSM1303467 het RNF17 6 week old adult testis rep1 RNAseq
GSM1303468 hom RNF17 6 week old adult testis rep1 RNAseq
GSM1303469 het RNF17 6 week old adult testis rep2 RNAseq
This SuperSeries is composed of the following SubSeries:
GSE53913 RNF17 referees ping-pong in mouse testes [RNAseq]
GSE53915 RNF17 blocks promiscuous activity of PIWI proteins in mouse testes [5'RACE]
GSE53917 RNF17 referees ping-pong in mouse testes [smallRNAseq]
Relations
BioProject PRJNA233962

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE53919_RAW.tar 23.7 Gb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp

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