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Series GSE53915 Query DataSets for GSE53915
Status Public on Dec 17, 2014
Title RNF17 blocks promiscuous activity of PIWI proteins in mouse testes [5'RACE]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary PIWI proteins and their associated piRNAs protect germ cells from the activity of mobile genetic elements. Two classes of piRNAs—primary and secondary—are defined by their mechanisms of biogenesis. Primary piRNAs are processed directly from transcripts of piRNA cluster loci, whereas secondary piRNAs are generated in an adaptive amplification loop, termed the ping-pong cycle. In mammals, piRNA populations are dynamic, shifting as male germ cells develop. Embryonic piRNAs consist of both primary and secondary species and are mainly directed toward transposons. In meiotic cells, the piRNA population is transposon-poor and largely restricted to primary piRNAs derived from pachytene piRNA clusters. The transition from the embryonic to the adult piRNA pathway is not well understood. Here we show that RNF17 shapes adult meiotic piRNA content by suppressing the production of secondary piRNAs. In the absence of RNF17, ping-pong occurs inappropriately in meiotic cells. Ping-pong initiates piRNA responses against not only transposons but also protein-coding genes and long noncoding RNAs, including genes essential for germ cell development. Thus, the sterility of Rnf17 mutants may be a manifestation of a small RNA-based autoimmune reaction.

Overall design Examination of 5'RACE in heterozygous and homozygous RNF17 adult testes or MIWI/MILI immunoprecipitates
Contributor(s) Wasik KA, Tam O, Hao Y, Falciatori I, Hammell M, Vagin VV, Hannon GJ
Citation(s) 26115953
Submission date Jan 08, 2014
Last update date May 15, 2019
Contact name Vasily Vagin
Organization name Cold Spring Harbor
Lab Hannon's
Street address 1 Bungtown Rd
City Cold Spring Harbor
State/province NY
ZIP/Postal code 11724
Country USA
Platforms (2)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (6)
GSM1303492 het_RNF17_ 5RACE_small and large500bp
GSM1303493 hom_RNF17_5RACE_small and large500bp
GSM1303494 het_RNF17_ MIWI_IP_5RACE
This SubSeries is part of SuperSeries:
GSE53919 RNF17 blocks promiscuous activity of PIWI proteins in mouse testes
BioProject PRJNA233961
SRA SRP035246

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Supplementary file Size Download File type/resource
GSE53915_RAW.tar 726.1 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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