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Series GSE53737 Query DataSets for GSE53737
Status Public on Nov 15, 2019
Title Oligodendrocyte Progenitor Cell Transcriptome in White Matter Stroke
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Tissue progenitors maintain the integrity of organ systems through aging and stress. The brain’s white matter regions experience ischemic lesions and age-dependent degeneration. Brain white matter contains progenitors, oligodendrocyte precursor cells (OPCs), which can repair some insults. The response of OPCs to white matter ischemia and aging is not known. We characterized the response of OPCs to white matter stroke using OPC reporter mice, cell migration tracking, OPC specific RNA sequencing, and mechanistic studies in candidate biochemical pathways in the aged brain. White matter stroke induces initial proliferation of local OPCs but blocks differentiation, shunting a portion into astrocytes. Candidate signaling pathways for this differentiation block including novel interactions of inhibin and matrilin-2 and new roles of NgR1 ligands following white matter stroke. Stroke induces inhibin expression in astrocytes and downregulates OPC matrilin-2 that contributes into OPC differentiation block. Antagonism of NgR1 ligands promotes OPC differentiation by attenuating the OPC astrocytic transformation and enhances functional recovery from stroke in aged animals.
Overall design RNA-sequencing of laser-capture oligodendrocyte precursor cell with a without white matter stroke
Contributor(s) Sozmen EG, Rosenzweig R, Machnicki M, Giger RJ, Coppola G, Hinman JD, Vinters H, Carmichael ST
Citation(s) 31591156
Submission date Dec 31, 2013
Last update date Feb 15, 2020
Contact name Giovanni Coppola
Phone 310-794-4172
Organization name UCLA
Department Psychiatry and Neurology
Lab Neurogenetics
Street address 1524 Gonda, 695 Charles Young Drive South
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (25)
GSM1299605 15d2
GSM1299606 15d3
GSM1299607 15d5
BioProject PRJNA232749
SRA SRP034855

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Supplementary file Size Download File type/resource
GSE53737_RPKM.txt.gz 2.0 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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