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Series GSE53638 Query DataSets for GSE53638
Status Public on Dec 23, 2014
Title Characterization of differentiating adipose cells by high-throughput single-cell RNA-Seq
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Directed differentiation of cells in vitro is a powerful approach for dissection of developmental pathways, disease modeling and regenerative medicine, but analysis of such systems are complicated by heterogeneous and asynchronous cellular responses to differentiation-inducing stimuli. To enable deep characterization of heterogeneous cell populations, we developed an efficient digital gene expression profiling protocol that enables surveying of mRNA in thousands of single cells at a time. We then applied this protocol to profile 11,116 cells collected during directed differentiation of human adipose-derived stem/stromal cells. The resulting data reveals the major axes of cell-to-cell variation within and between time points and suggests a link between incomplete adipogenesis in vitro and adipocyte dysfunction in vivo.
Overall design High-throughput single cell RNA-seq method applied to human adipose tissue-derived stromal/stem cells during differentiation towards an adipogenic fate
Contributor(s) Soumillon M, Cacchiarelli D, Semrau S, van Oudenaarden A, Mikkelsen TS
Citation Characterization of directed differentiation by high-throughput single-cell RNA-Seq. Magali Soumillon, Davide Cacchiarelli, Stefan Semrau, Alexander van Oudenaarden, Tarjei S Mikkelsen. bioRxiv. Mar 5, 2014.
Submission date Dec 24, 2013
Last update date May 15, 2019
Contact name Magali Soumillon
Organization name Harvard University, HSCRB
Street address Divinity Avenue 7
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
Platforms (2)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (52)
GSM1297624 D1T0A
GSM1297625 D1T0B
GSM1297626 D1T0C
BioProject PRJNA232531
SRA SRP034712

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE53638_D1_UMI.dat.gz 10.0 Mb (ftp)(http) DAT
GSE53638_D2_UMI.dat.gz 2.9 Mb (ftp)(http) DAT
GSE53638_D3_Bulk_UMI.dat.gz 225.7 Kb (ftp)(http) DAT
GSE53638_D3_Lipid_UMI.dat.gz 3.4 Mb (ftp)(http) DAT
GSE53638_D3_UMI.dat.gz 5.1 Mb (ftp)(http) DAT
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Raw data are available in SRA
Processed data are available on Series record

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