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Series GSE53528 Query DataSets for GSE53528
Status Public on Aug 12, 2015
Title Myogeneic differentiation in Rb1 or Kdm5a/Jarid1a/Rbp2 deficient mouse embryonic fibroblasts.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Cells lacking Rb1 are deficient in differentiation. Loss of Kdm5a rescues myogenic differentiation, as judged by appearance of morphologically normal myotubes that display expression of late markers of differentiation. In order to better understand how Kdm5a loss rescues differentiation, we induced mouse embryonic fibroblasts (MEFs) of different genotypes to undergo myogenic differentiation and analyzed gene expression changes in wild-type, Kdm5a-/-, Rb1-/- and Kdm5a-/-; Rb1-/- cells. Rb1-/- cells stained single nucleated, did not exhibit morphological changes and increased expression of the myogenic marker MYHC. Except for Rb1-/- cells, all other cells were undergoing successful convertion into aligned multinucleated myotubes and were MYHC-positive. We obtained purified populations of myotubes for the wild-type and Kdm5a-/-; Rb1-/- cells.
Overall design RNA-seq analysis of gene expression in Rb1 or Kdm5a deficient MEFs that were induced for myogenic differentiation.
Contributor(s) Islam A, Benevolenskaya E
Citation(s) 26314709
Submission date Dec 19, 2013
Last update date May 15, 2019
Contact name Elizaveta V Benevolenskaya
Phone 312-413-8947
Organization name University of Illinois at Chicago
Department Biochemistry and Molecular Genetics
Street address 900 S. Ashland Ave.
City Chicago
State/province IL
ZIP/Postal code 60607
Country USA
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (12)
GSM1295547 WT rep1
GSM1295548 WT rep2
GSM1295549 Kdm5a KO rep1
BioProject PRJNA232284
SRA SRP034644

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Supplementary file Size Download File type/resource
GSE53528_Normalized_ReadCount.txt.gz 1.7 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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