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Status |
Public on Feb 12, 2015 |
Title |
Circulating microRNAs are candidate markers of heart failure and differentiate between heart failure with reduced vs preserved left ventricular ejection fraction. |
Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human alphaherpesvirus 2; Human betaherpesvirus 5; Murid betaherpesvirus 1; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Merkel cell polyomavirus; Mus musculus cytomegalovirus 2; Betapolyomavirus hominis; Betapolyomavirus macacae |
Sample organism |
Homo sapiens |
Experiment type |
Non-coding RNA profiling by array
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Summary |
In this study, we compared the expression profiles of circulating miRNAs in blood samples from controls and patients with heart ailment. Subject with no past history of heart failure/disease are considered as controls. The patients were classified according to the percentage of left ventricular ejection fraction. Patients were grouped as heart failure with reduced (hfREF) and preserved (hfPEF) left ventricular ejection fraction. Employing miRNA microarray, we identified 'signature miRNAs' in peripheral blood samples that distinguished Heart failure from the non-heart failure controls, as well as those of hfREF and hfPEF groups.
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Overall design |
Total RNA (plus miRNAs) was isolated using a modification of the RiboPure™-Blood kit from Ambion (Austin,TX) according to the manufacturer’s protocol. The concentration of total RNA and integrity were determined by using Nano-Drop ND-1000 Spectrophotometry (NanoDrop Tech, Rockland, Del) and gel electrophoresis respectively.
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Contributor(s) |
Jeyaseelan K, Sepramaniam S, Armugam A, Karolina D, Lim K, Lam CS, Richards MA |
Citation(s) |
25619197 |
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Submission date |
Dec 18, 2013 |
Last update date |
May 10, 2016 |
Contact name |
Kandiah Jeyaseelan |
E-mail(s) |
erijeya@nus.edu.sg
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Organization name |
National University of Singapore
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Department |
Biochemistry
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Street address |
8 Medical Drive
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City |
Singapore |
ZIP/Postal code |
117597 |
Country |
Singapore |
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Platforms (3) |
GPL11434 |
miRCURY LNA microRNA Array, 6th generation - hsa, mmu & rno |
GPL18066 |
Exiqon miRCURY LNA microRNA array, 7th generation [miRBase v18, condensed human_miRNA version] |
GPL18067 |
miRCURY LNA microRNA Array, v.11.0 - hsa, mmu & rno [human miRNA ID version] |
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Samples (90)
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Relations |
BioProject |
PRJNA231992 |