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Series GSE53437 Query DataSets for GSE53437
Status Public on Feb 12, 2015
Title Circulating microRNAs are candidate markers of heart failure and differentiate between heart failure with reduced vs preserved left ventricular ejection fraction.
Platform organisms Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human alphaherpesvirus 2; Human betaherpesvirus 5; Murid betaherpesvirus 1; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Merkel cell polyomavirus; Mus musculus cytomegalovirus 2; Betapolyomavirus hominis; Betapolyomavirus macacae
Sample organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary In this study, we compared the expression profiles of circulating miRNAs in blood samples from controls and patients with heart ailment. Subject with no past history of heart failure/disease are considered as controls. The patients were classified according to the percentage of left ventricular ejection fraction. Patients were grouped as heart failure with reduced (hfREF) and preserved (hfPEF) left ventricular ejection fraction. Employing miRNA microarray, we identified 'signature miRNAs' in peripheral blood samples that distinguished Heart failure from the non-heart failure controls, as well as those of hfREF and hfPEF groups.
 
Overall design Total RNA (plus miRNAs) was isolated using a modification of the RiboPure™-Blood kit from Ambion (Austin,TX) according to the manufacturer’s protocol. The concentration of total RNA and integrity were determined by using Nano-Drop ND-1000 Spectrophotometry (NanoDrop Tech, Rockland, Del) and gel electrophoresis respectively.
 
Contributor(s) Jeyaseelan K, Sepramaniam S, Armugam A, Karolina D, Lim K, Lam CS, Richards MA
Citation(s) 25619197
Submission date Dec 18, 2013
Last update date May 10, 2016
Contact name Kandiah Jeyaseelan
E-mail(s) erijeya@nus.edu.sg
Organization name National University of Singapore
Department Biochemistry
Street address 8 Medical Drive
City Singapore
ZIP/Postal code 117597
Country Singapore
 
Platforms (3)
GPL11434 miRCURY LNA microRNA Array, 6th generation - hsa, mmu & rno
GPL18066 Exiqon miRCURY LNA microRNA array, 7th generation [miRBase v18, condensed human_miRNA version]
GPL18067 miRCURY LNA microRNA Array, v.11.0 - hsa, mmu & rno [human miRNA ID version]
Samples (90)
GSM1293460 Control1 [set 1]
GSM1293461 hfREF1 [set 1]
GSM1293462 hfREF2 [set 1]
Relations
BioProject PRJNA231992

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE53437_RAW.tar 70.0 Kb (http)(custom) TAR
GSE53437_non_normalized_set1.txt.gz 811.8 Kb (ftp)(http) TXT
GSE53437_non_normalized_set2.txt.gz 783.2 Kb (ftp)(http) TXT
GSE53437_non_normalized_set3.txt.gz 462.0 Kb (ftp)(http) TXT
Processed data included within Sample table

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