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Series GSE53349 Query DataSets for GSE53349
Status Public on Oct 01, 2015
Title CD8+ T cells during acute viral respiratory infection are uniquely differentiated and regulated by multiple inhibitory receptors
Organism Mus musculus
Experiment type Expression profiling by array
Summary Acute viral infection typically generates functional effector CD8+ T cells that aid in pathogen clearance. However, during acute viral lower respiratory infection (LRI), lung CD8+ T cells are functionally impaired and do not optimally control viral replication, while spleen CD8+ T cells specific for the same viral epitopes remain fully functional. To better understand the mechanisms governing lung CD8+ T cell impairment, we used flow cytometry to sort anti-viral CD8+ T cells during viral LRI. Lung and spleen cells were stained with MHC-class I tetramers representing the immunodominant anti-viral CD8+ T cell epitope. We then sorted to high purity: naïve CD8+ T cells, spleen epitope-specific CD8+ T cells, lung epitope-specific CD8+ cells and secondary infection lung epitope-specific CD8+ T cells. We then performed a genome wide transcriptional analysis of these cells to characterize the gene expression profile of lung CD8+ T cell impairment.
Overall design Epitope-specific lung and spleen CD8+ T cells were obtained using flow cytometric sorting of MHC-class I tetramer stained CD8+ T cells at day 7 after human metapneumovirus (HMPV) infection. Tetramers were specific for the immunodominant M195 epitope (derived from the M protein of HMPV) in B6-Kb0Db0;B7.2 transgenic (B7tg) mice. Naïve CD44-CD62L+ CD8+ T cells were also obtained from the spleen. In a separate group, mice were primed with M195 peptide-pulsed LPS-matured bone marrow-derived dendritic cells, challenged with HMPV two weeks later, and secondary M195-specific CD8+ T cells were obtained at day 7 post-challenge.
Contributor(s) Erickson JJ, Lu P, Gilchuk P, Joyce S, Shyr Y, Williams JV
Citation(s) 26401005
Submission date Dec 16, 2013
Last update date Apr 18, 2017
Contact name Pengcheng Lu
Organization name Vanderbilt University
Street address 2200 Pierce Avenue
City Nashville
State/province TN
ZIP/Postal code 37232
Country USA
Platforms (1)
GPL11533 [MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version]
Samples (12)
GSM1289642 Naïve_1
GSM1289643 Naïve_2
GSM1289644 Naïve_3
BioProject PRJNA231771

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Supplementary file Size Download File type/resource
GSE53349_RAW.tar 46.6 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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