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Series GSE53220 Query DataSets for GSE53220
Status Public on Feb 11, 2014
Title Genomic analyses reveal miR-137 broad impact on genes associated with malignant transformation and neuronal differentiation in glioblastoma cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary miR-137 plays critical roles in the nervous system and tumor development. An increase in its expression is required for neuronal differentiation while its reduction is implicated in gliomagenesis. To evaluate the potential of miR-137 in glioblastoma therapy, we conducted genome-wide target mapping in glioblastoma cells by measuring levels of associations between PABP and mRNAs in cells transfected with miR-137 mimics vs. controls via RIPSeq. Impact on mRNA levels was also measured by RNASeq. By combining the results of both experimental approaches, 1468 genes were determined to be negatively impacted by miR-137; among them, 595 (40%) contain miR-137 predicted sites. The most relevant targets include oncogenic proteins and players in neurogenesis like c-KIT, YBX1, AKT2, CDC42, CDK6 and TGFβ2. Interestingly, we observed that several identified miR-137 targets are also predicted to be regulated by miR-124, miR-128 and miR-7, which are equally implicated in neuronal differentiation and gliomagenesis. We suggest that the concomitant increase of these four miRNAs in neuronal stem cells or their repression in tumor cells could produce a robust regulatory effect with major consequences to neuronal differentiation and tumorigenesis.
Overall design Identification of genes affected by miR137 transfection via RIP-Seq and RNA-Seq in U251 and U343 cells
Contributor(s) Uren PJ, Vo DT, Penalva LO
Citation(s) 24465609
Submission date Dec 11, 2013
Last update date May 15, 2019
Contact name Philip James Uren
Organization name University of Southern California
Street address 1050 Childs Way, RRI201
City Los Angeles
State/province CA
ZIP/Postal code 90089-2910
Country USA
Platforms (1)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
Samples (16)
GSM1287857 U251 RNASeq control transfection Rep1
GSM1287858 U251 RNASeq control transfection Rep2
GSM1287859 U251 RNASeq miR137 transfection Rep1
BioProject PRJNA231155
SRA SRP033711

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Supplementary file Size Download File type/resource
GSE53220_RAW.tar 5.0 Mb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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