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Series GSE53207 Query DataSets for GSE53207
Status Public on Jan 21, 2016
Title H2A.Z.1 mono-ubiquitylation antagonizes BRD2 to maintain poised chromatin in ESCs [RNA-seq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Histone variant H2A.Z occupies the promoters of active and poised, bivalent genes in ESCs to regulate developmental programs, yet how it contributes to these contrasting states is poorly understood. Here, we investigate the function of H2A.Z.1 mono-ubiquitylation (H2A.Z.1ub) by mutation of the PRC1 target residues (H2A.Z.1K3R3). We show that H2A.Z.1K3R3 is properly incorporated at target promoters in murine ESCs (mESCs), however, loss of mono-ubiquitylation leads to de-repression of bivalent genes, loss of Polycomb binding, and to faulty lineage commitment. Using quantitative proteomics, we find that tandem bromodomain proteins, including the BET family member Brd2, are enriched in H2A.Z.1 chromatin. We further show that Brd2 is gained at de-repressed promoters in H2A.Z.1K3R3 mESCs whereas Brd2 inhibition restores gene silencing at these sites. Together, our study reveals an antagonistic relationship between H2A.Z.1ub and Brd2 to regulate the transcriptional balance at bivalent genes to enable proper execution of developmental programs.
Overall design RNA-Seq analysis on mouse embryonic stem cells harboring H2A.Z or H2A.Z.K3R3 (3 C-terminal lysines mutated to arginines) tagged with YFP, in the presence of a knockdown hairpin targeting the endogenous H2A.Z transcript.
Contributor(s) Surface LE, Fields PA, Boyer LA
Citation(s) 26804911
Submission date Dec 11, 2013
Last update date May 15, 2019
Contact name Laurie A Boyer
Phone 617 324-3335
Organization name Massachusetts Institute of Technology
Department Biology
Lab Boyer
Street address 77 Massachusetts Avenue
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (3)
GSM1287701 H2AZ_WT_ESC_Rep1
GSM1287702 H2AZ_WT_ESC_Rep2
GSM1287703 H2AZ_K3R3_ESC
This SubSeries is part of SuperSeries:
GSE53208 H2A.Z.1 mono-ubiquitylation antagonizes BRD2 to maintain poised chromatin in ESCs
BioProject PRJNA231112
SRA SRP033699

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Supplementary file Size Download File type/resource
GSE53207_RNASeq_Expression.txt.gz 699.9 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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