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Series GSE53076 Query DataSets for GSE53076
Status Public on Dec 07, 2013
Title Genome-wide identification of regulatory elements in preSertoli cells based on DNaseI hypersensitivity
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary Identifying transcriptional networks active during development has been hindered by the slow pace at which regulatory elements are identified, and by the difficulty in purifying cells from developing tissues. We used DNaseI-seq to identify functional regulatory elements in purified fetal preSertoli cells and uncovered thousands of preSertoli-specific DNaseI hypersensitive sites (DHSs) that are likely to activate or repress genes required for Sertoli cell development and function. To demonstrate their functional significance, we validated the preSertoli-specific enhancer activity of a DHS located 50 kb upstream of the Wt1 gene. This genome-wide data will guide many future studies into gene-regulatory mechanisms that operate in the fetal gonad and may lead to the identification of non-coding mutations leading to DSDs.
Overall design One biological replicate was analyzed for each of the eight mouse samples using DNase-seq. Three biological replicates were analyzed for RNA-seq. Two biological replicates for ChIP-seq.
Contributor(s) Shibata Y, Maatouk DM
Citation(s) 28087634
Submission date Dec 06, 2013
Last update date May 15, 2019
Contact name Danielle Maatouk
Organization name Northwestern University
Street address 303 E. Superior St., Lurie 7-250
City Chicago
ZIP/Postal code 60611
Country USA
Platforms (2)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (15)
GSM1281688 E15.5_Sox9CFP
GSM1281689 ES_JM8_B3T1HS
GSM1281690 Fibroblast_M_B3T1
BioProject PRJNA230803
SRA SRP033562

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE53076_K27ac_E13Sertoli.txt.gz 190.8 Kb (ftp)(http) TXT
GSE53076_RAW.tar 24.2 Gb (http)(custom) TAR (of BED, BIGWIG, TXT)
GSE53076_RNA-seq_E15Sertoli.txt.gz 530.2 Kb (ftp)(http) TXT
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Raw data are available in SRA
Processed data provided as supplementary file

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