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| Status |
Public on Jul 03, 2014 |
| Title |
Regulation of Ptch1 expression in the limb |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Ptch1 is a critical negative feedback regulator of the Hedgehog signaling and is upregulated in response to pathway activation. How tissue specific responses are mediated remains unknown. Here, we performed ChIP-seq analysis for epitope-tagged endogenous Gli3 protein to identify limb-specific binding regions within the 500 kb region surrounding Ptch1.
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| |
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| Overall design |
ChIP was carried out using an endogenously FLAG-tagged Gli3 protein.
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| |
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| Contributor(s) |
McMahon AP, Peterson KA |
| Citation(s) |
24990743, 27146892 |
| |
| Submission date |
Dec 03, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Kevin Peterson |
| E-mail(s) |
kevin.peterson@med.usc.edu
|
| Phone |
323-442-8077
|
| Organization name |
Eli and Edythe Broad-CIRM Center for Regenerative Medicine, University of Southern California Keck School of Medicine
|
| Department |
Department of Stem Cell Biology and Regenerative Medicine
|
| Lab |
Andrew McMahon
|
| Street address |
1425 San Pablo St.
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (2) |
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| Relations |
| BioProject |
PRJNA230497 |
| SRA |
SRP033452 |
