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Series GSE52935 Query DataSets for GSE52935
Status Public on Mar 30, 2015
Title SETX attenuates the antiviral innate immune response and controls viral biogenesis (GRO-Seq)
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The innate immune response is the first line of defense against pathogens, and factors that control this cellular response represent targets for treating both infectious and inflammatory diseases. Here, we reveal a novel role for the human helicase SETX, also previously implicated in amyotrophic lateral sclerosis (ALS4) and ataxia with oculomotor apraxia (AOA2), in controlling the magnitude of the antiviral response. Cells depleted for SETX and AOA2 patient-derived SETX-null cells show increased expression of antiviral mediators in response to infection. Mechanistically, this effect is achieved through SETX-mediated inhibition of RNAPII transcription of antiviral genes, and depends on SETX helicase activity. Our results suggest that SETX helps maintain the delicate balance between controlling viral infection and avoiding the potentially detrimental effects of an excessive antiviral response. More broadly, the observation that SETX can regulate the transcriptional activity of specific genes may have important implications for disorders where SETX function is compromised.
Overall design A549 cells that were transfected with either control non-targeting or SETX-specific siRNAs were infected with the Influenza A virus (A/PR/8/34(ΔNS1) strain) at a multiplicity of infection (MOI) of 3 for 4 hours. Nuclei were then extracted and used to prepare Global Run-On Sequencing (GRO-seq) libraries.
Contributor(s) Miller MS, Rialdi A, Ho JS, Tilove M, Martinez-Gil L, Moshkina NP, Peralta Z, Noel J, Melegari C, Maestre A, Mitsopoulos P, Madrenas J, Heinz S, Benner C, Young JA, Feagins AR, Basler CF, Fernandez-Sesma A, Bequerel OJ, Lavin MF, van Bakel H, Marazzi I
Citation(s) 25822250
Submission date Dec 03, 2013
Last update date May 15, 2019
Contact name Ivan Marazzi
Organization name Icahn School of Medicine at Mount Sinai
Department Department of Microbiology
Street address 1468 Madison Avenue
City New York
ZIP/Postal code 10029
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (4)
GSM1278354 siCtrl-GRO-t0
GSM1278355 siCtrl-GRO-t4
GSM1278356 siSetx-GRO-t0
This SubSeries is part of SuperSeries:
GSE52937 Senataxin suppresses the antiviral transcriptional response and controls viral biogenesis
BioProject PRJNA230478
SRA SRP033442

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Supplementary file Size Download File type/resource
GSE52935_RAW.tar 55.8 Mb (http)(custom) TAR (of BEDGRAPH)
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Raw data are available in SRA
Processed data provided as supplementary file

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