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Series GSE5287 Query DataSets for GSE5287
Status Public on Aug 01, 2007
Title Prediction of response and survival following chemotherapy in patients with advanced bladder cancer
Organism Homo sapiens
Experiment type Expression profiling by array
Cisplatin-containing chemotherapy is the standard of care for patients with locally advanced and metastatic transitional cell carcinoma of the urothelium.
The response rate is around 50% and tumor derived molecular prognostic markers are needed for estimation of response and survival.
Affymetrix GeneChip expression profiling was carried out using tumor material from 30 patients. A set of genes having an expression with high correlation to survival time after chemotherapy was identified. Two of these genes were selected for validation by immunohistochemistry (IHC) in tumor tissue from 149 cisplatin treated patients having complete follow-up data.
Fifty-five differentially expressed genes correlated significantly to survival time. Two of these (Emmprin and Survivin) were validated using IHC, and multivariate analysis (n=145) identified Emmprin expression (hazard ratio 2.38; p<0.0001) and Survivin expression (hazard ratio 2.34; p<0.0001) as independent prognostic markers for poor outcome, together with the presence of visceral metastases (hazard ratio 2.72; p<0.0001). In the good prognosis group of patients without visceral metastases, both markers showed significant discriminating power as supplemental risk factors (p<0.0001). Within this group of patients, the subgroups of patients with no positive, one positive or two positive IHC stainings (Emmprin and Survivin) had estimated 5-year survival rates (+- SE) of 35.6+-?%, 6.3+-?%, and 0+?%, respectively. Response to chemotherapy could also be predicted with an OddsRatio of 4.60 (2.13-9.93) and 2.59 (1.25-5.38) for Emmprin and Survivin respectively.
Emmprin and Survivin proteins were identified as strong independent prognostic predictors for response and survival after cisplatin-containing chemotherapy in patients with bladder cancer.
Keywords: Analysis of gene expression differences between responders and non-responders to chemotherapy
Overall design Tumors from 30 patients with advanced bladder cancer used in the study. A SAM analysis was used for identifying genes co-varying with treatment response.
Citation(s) 17671123
Submission date Jul 11, 2006
Last update date Aug 10, 2018
Contact name Lars Dyrskjot
Organization name Aarhus University Hospital, Skejby
Department Department of Molecular Medicine
Street address Brendstrupgaardsvej
City Aarhus N
ZIP/Postal code 8200
Country Denmark
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (30)
GSM119491 217-16
GSM119492 472-1
GSM119493 523-1
BioProject PRJNA96407

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Supplementary file Size Download File type/resource
GSE5287_RAW.tar 97.9 Mb (http)(custom) TAR (of CEL)

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