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| Status |
Public on Dec 22, 2013 |
| Title |
Gene expression profiling to identify genes that are differentially expressed between murine MHCIIlo and MHCIIhi dendritic cell subpopulations [MHCIIhi vs. MHCIIlo] |
| Platform organism |
Mus musculus |
| Sample organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Hematopoietic progenitors from mouse bone marrow were differentiated into dendritic cells for 5 days with GM-CSF and IL-4. On day 6, MHCIIhi and MHCIIlo BMDC subpopulations were sorted to purify by flow cytometry. RNA was extracted and processed for microarray analysis.
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| Overall design |
Two condition experiment; Biological replicates: 4 replicates per condition.
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| Contributor(s) |
Modrusan Z, Vander Lugt B, Hackney JA, Khan AA, Agrawal S, Lesch J, Zhou M, Lee WP, Park S, Xu M, DeVoss J, Spooner CJ, Chalouni C, Delamarre L, Mellman I, Singh H |
| Citation(s) |
24362890 |
| |
| Submission date |
Nov 26, 2013 |
| Last update date |
Jan 12, 2017 |
| Contact name |
Jason A Hackney |
| E-mail(s) |
hackney.jason@gene.com
|
| Organization name |
Genentech, Inc.
|
| Department |
Department of Bioinformatics and Computational Biology
|
| Street address |
1 DNA Way
|
| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
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| Platforms (1) |
| GPL7202 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version) |
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| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE52773 |
Transcriptional programming of dendritic cells for enhanced major histocompatibility complex class II antigen presentation |
|
| Relations |
| BioProject |
PRJNA231193 |
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