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Series GSE52524 Query DataSets for GSE52524
Status Public on May 19, 2016
Title Network Biology of the Skin [1]
Organism Mus musculus
Experiment type Expression profiling by array
Summary Gene expression levels in normal tissues can differ substantially between individuals, due to inherited polymorphisms acting in cis or trans. Analysis of this variation across a population of genetically distinct individuals allows us to visualize a network of co-expressed genes under normal homeostatic conditions, and the consequences of perturbation by tissue damage or disease development. Here, we explore gene expression networks in normal adult skin from 470 genetically unique mice, and demonstrate the dependence of the architecture of signaling pathways on skin tissue location (dorsal or tail skin) and perturbation by induction of inflammation or tumorigenesis. Gene networks related to specific cell types, as well as signaling pathways including Sonic Hedgehog (Shh), Wnt, Lgr family stem cell markers, and keratins differed at these tissue sites, suggesting mechanisms for the differential susceptibility of dorsal and tail skin to development of skin diseases and tumorigenesis. The Pten tumor suppressor gene network is extensively rewired in premalignant tumors compared to normal tissue, but this response to perturbation is lost during malignant progression. We present a software package for eQTL network analysis and demonstrate how network analysis of whole tissues provides insights into interactions between cell compartments and signaling molecules.
Overall design A backcross was generated using male Mus spretus and female FVB/N mice; female F1 hybrids were mated with male FVB/N mice. Mice were aged to 8 weeks and sacrificed. Shaved dorsal skin and tail skin were snap frozen.
Contributor(s) Quigley DA, Balmain A
Citation(s) 27425619
Submission date Nov 19, 2013
Last update date Apr 18, 2017
Contact name David Quigley
Organization name UCSF
Department Helen Diller Comprehensive Cancer Center
Lab Ashworth Lab
Street address 1450 Third St. Room 207
City San Francisco
State/province CA
ZIP/Postal code 94158
Country USA
Platforms (1)
GPL11533 [MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version]
Samples (196)
GSM1268711 back-1000
GSM1268712 tail-1000
GSM1268713 back-1001
This SubSeries is part of SuperSeries:
GSE52650 Gene expression architecture of mouse dorsal and tail skin reveals functional differences in inflammation and cancer
BioProject PRJNA229159

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE52524_RAW.tar 792.7 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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