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Series GSE52501 Query DataSets for GSE52501
Status Public on Apr 15, 2014
Title The nucleosomal barrier to promoter escape by RNA Polymerase II is overcome by the chromatin remodeler Chd1 [HiSeq]
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We use a metabolic labeling strategy for directly measuring nucleosome turnover to determine the role of chromatin remodeler Chd1 on chromatin dynamics in mouse embryonic fibroblasts expressing with wildtype-Chd1 and dominant negative K510R-Chd1. We find that expression of K5 10R-Chd1 decreases nucleosome turnover at the promoter and increases turnover within the gene body.
Overall design 10 Illumina sequencing samples and 4 Nimblegen array samples
Contributor(s) Skene PJ, Hernandez AE, Groudine M, Henikoff S
Citation(s) 24737864
Submission date Nov 19, 2013
Last update date May 15, 2019
Contact name Jorja Henikoff
Phone 206-667-4850
Organization name Fred Hutchinson Cancer Research Center
Department Basic Sciences
Lab Henikoff
Street address 1100 Fairview AV N, A1-162
City Seattle
State/province WA
ZIP/Postal code 98109-1024
Country USA
Platforms (2)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (10)
GSM1267944 K510R_MEFs_FLAG_ChIP_(20120817_6_12)
GSM1267945 K510R_MEFs_MNase_(20120817_4_12)
GSM1267946 K510R_MEFs_N20_ChIP_(20120817_5_6)
This SubSeries is part of SuperSeries:
GSE52349 The nucleosomal barrier to promoter escape by RNA Polymerase II is overcome by the chromatin remodeler Chd1
BioProject PRJNA229126
SRA SRP033123

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE52501_RAW.tar 8.9 Gb (http)(custom) TAR (of BED, BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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