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Series GSE52497 Query DataSets for GSE52497
Status Public on Jun 12, 2014
Title A crucial role of the ubiquitously expressed transcription factor Sp1 at early stages of hematopoietic specification [array]
Organism Mus musculus
Experiment type Expression profiling by array
Summary Mammalian development is regulated by the interplay of tissue-specific and ubiquitously expressed transcription factors, such as Sp1. Sp1 knock-out mice die in utero with multiple phenotypic aberrations, but the underlying molecular mechanism of this differentiation failure has been elusive. Here we used conditional knock-out mice as well as the differentiation of mouse ES cells as a model to address this issue. To this end we examined differentiation potential, global gene expression patterns and Sp1 target regions in Sp1 wild-type and deficient cells representing different stages of hematopoiesis. Sp1-/- cells progress through most embryonic stages of blood cell development but cannot complete terminal differentiation. For most Sp1 target and non-target genes, gene expression is unaffected by Sp1 inactivation. However, Cdx and multiple Hox genes are stage-specific targets of Sp1 and are down-regulated at an early stage. As a consequence, expression of genes involved in hematopoietic specification are progressively deregulated, highlighting the regulatory hierarchy of hematopoietic specification. Our work demonstrates that the early absence of active Sp1 sets a cascade in motion that culminates in a failure of terminal hematopoietic differentiation and emphasizes the role of ubiquitously expressed transcription factors for tissue-specific gene regulation.
Overall design Microarray expression data, 16 arrays with 2 independent biological replicates (8 arrays wildtype and 8 arrays knock out) obtained from differentiation of ES cells to study the transcription factor Sp1 activity at early stages of early hematopoietic specification.
Contributor(s) Gilmour J, Assi SA, Jaegle U, Kulu D, van de Werken H, Clarke D, Westhead DR, Philipsen S, Bonifer C
Citation(s) 24850855
Submission date Nov 19, 2013
Last update date Jul 19, 2017
Contact name Salam Adli Assi
Organization name University of Birmingham
Department Institute for Cancer and Genomic Sciences
Street address IBR
City Birmingham
ZIP/Postal code B15 2TT
Country United Kingdom
Platforms (1)
GPL13912 Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version)
Samples (16)
GSM1267915 E14 hemogenic endothelium I (HE1) replicate1
GSM1267916 E14 hemogenic endothelium II (HE2) replicate1
GSM1267917 E14 progenitor replicate1
This SubSeries is part of SuperSeries:
GSE52499 A crucial role of the ubiquitously expressed transcription factor Sp1 at early stages of hematopoietic specification
BioProject PRJNA229110

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Supplementary file Size Download File type/resource
GSE52497_RAW.tar 58.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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