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Series GSE52437 Query DataSets for GSE52437
Status Public on Dec 18, 2013
Title Switch Enhancer Elements Control Cell Fate in Human Embryonic Stem Cells (ChIP-Seq)
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary A small toolkit of morphogens is used repeatedly to direct development, raising the question of how context dictates interpretation of the same cue. One example is the TGFβ pathway that in human embryonic stem cells fulfills two opposite functions: pluripotency maintenance and mesendoderm (ME) specification. Using proteomics coupled to analysis of genome occupancy, we uncover a regulatory complex comprised of transcriptional effectors of the Hippo pathway (TAZ/YAP/TEAD), the TGFβ pathway (SMAD2/3) and the pluripotency regulator OCT4 (TSO). TSO collaborates with NuRD repressor complexes to buffer pluripotency gene expression, while suppressing ME genes. Importantly, the SMAD DNA binding partner FOXH1, a major specifier of ME, is found near TSO elements and upon fate specification we show that TSO is disrupted with subsequent SMAD-FOXH1 induction of ME. These studies define switch enhancer elements and provide a framework to understand how cellular context dictates interpretation of the same morphogen signal in development.
Overall design ChIP experiment, a total of 8 samples: 4 samples DMSO treated (Input ctr, IgG ctr, SMAD2 precipitation, TEAD4 precipitation), 4 samples SB431542 (Input ctr, IgG ctr, SMAD2 precipitation, TEAD4 precipitation)
Contributor(s) Beyer TA, Wrana JL
Citation(s) 24332857
Submission date Nov 15, 2013
Last update date May 15, 2019
Contact name Tobias Andreas Beyer
Phone +1 416 586 4800
Organization name Mount Sinai Hospital
Department Lunenfeld-Tannenbaum Research Institute
Lab Wrana Lab
Street address 600 University Ave
City Toronto
State/province ONT
ZIP/Postal code M5G 1X5
Country Canada
Platforms (1)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
Samples (8)
GSM1266811 DMSO Input DNA
GSM1266812 SB431542 Input DNA
GSM1266813 DMSO IgG ChIP
This SubSeries is part of SuperSeries:
GSE52440 Switch Enhancer Elements Control Cell Fate in Human Embryonic Stem Cells
BioProject PRJNA228926
SRA SRP033079

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Supplementary file Size Download File type/resource
GSE52437_Smad_DMSO.bed.gz 3.2 Mb (ftp)(http) BED
GSE52437_TEAD4_DMSO.bed.gz 2.2 Mb (ftp)(http) BED
GSE52437_TEAD4_SB.bed.gz 2.3 Mb (ftp)(http) BED
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Raw data are available in SRA
Processed data are available on Series record

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