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Series GSE52428 Query DataSets for GSE52428
Status Public on Nov 16, 2013
Title Host gene expression signatures of influenza A H1N1 and H3N2 virus infection in adults
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Diagnosis of influenza A infection is currently based on clinical symptoms and pathogen detection. Use of host peripheral blood gene expression data to classify individuals with influenza A virus infection represents a novel approach to infection diagnosis
We used microarrays to assay peripheral blood gene expression at baseline and every 8 hours for 7 days following intranasal influenza A H1N1 or H3N2 inoculation in healthy volunteers. We determined groups of coexpressed genes that classified symptomatic influenza infection. We then tested this gene expression classifier in patients with naturally acquired respiratory illness.
Overall design We experimentally inoculated healthy volunteers with intranasal influenza A H1N1 and H3N2. Symptoms were documented and peripheral blood samples drawn into PAXgene RNA tubes for RNA isolation. We further enrolled patients presenting to the Emergency Department with naturally acquired respiratory illness, and documented symptoms and collected PAXgene RNA samples for RNA isolation.
Contributor(s) Woods CW, Ginsburg GS
Citation(s) 23326326
Submission date Nov 15, 2013
Last update date Nov 30, 2020
Contact name Geoffrey S Ginsburg
Organization name Duke University Medical Center
Department Genomic Medicine
Lab Ginsburg
Street address 101 Science Dr, CIEMAS 2111, Box 3382
City Durham
State/province NC
ZIP/Postal code 27708
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (649)
GSM1265874 Influenza challenge sample at 5 hours from subject H3N2_001
GSM1265875 Influenza challenge sample at Baseline from subject H3N2_001
GSM1265876 Influenza challenge sample at Pre-Challenge Baseline from subject H3N2_001
BioProject PRJNA228916

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE52428_RAW.tar 1.4 Gb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file

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