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Series GSE5229 Query DataSets for GSE5229
Status Public on Jul 08, 2006
Title Cross-regulation between Notch and p63 in keratinocyte commitment to differentiation
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by array
Summary Notch signaling promotes commitment of keratinocytes to differentiation and suppresses tumorigenesis. p63, a p53 family member, has been implicated in establishment of the keratinocyte cell fate and/or maintenance of epithelial self-renewal. Here we show that p63 expression is suppressed by Notch1 activation in both mouse and human keratinocytes through a mechanism independent of cell cycle withdrawal and requiring down-modulation of selected interferon-responsive genes, including IRF7 and/or IRF3. In turn, elevated p63 expression counteracts the ability of Notch1 to restrict growth and promote differentiation. p63 functions as a selective modulator of Notch1-dependent transcription and function, with the Hes-1 gene as one of its direct negative targets. Thus, a complex cross-talk between Notch and p63 is involved in the balance between keratinocyte self-renewal and differentiation.
Keywords: Notch1, p63, keratinocyte differentiation, gene expression profiling
Overall design PolyA+ mRNA (2-5 ug) from cells infected with the Ad GFP and Ad-NotchIC viruses was used as template for double stranded cDNA preparations with a T7-(dT)24 oligonucleotide primers for the first strand reaction. The resulting cDNA was used for preparation of biotin-labeled antisense cRNA and further used for hybridization to Affymetrix U74A or U95A “gene chips” according to the manufacturer’s recommendation.
Contributor(s) Nguyen BC, Wang J, Devgan V, Lefort K, Buck C, Antonini D, Kitajewski J, Chiorino G, Missero C, Dotto GP
Citation(s) 16618808
Submission date Jul 05, 2006
Last update date Jan 08, 2019
Contact name G.Paolo Dotto
Phone 011-41-21-692 5851
Organization name Lausanne University
Department Biochemistry
Lab Dotto
Street address 153 Chemin de Bovaress
City Epalinges
State/province VD
ZIP/Postal code 1066
Country Switzerland
Platforms (2)
GPL339 [MOE430A] Affymetrix Mouse Expression 430A Array
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (4)
GSM108473 Human Keratinocytes Ad-Notch1
GSM108474 Human Keratinocytes Ad-GFP
GSM108476 Mouse Keratinocytes Ad-Notch1
BioProject PRJNA96431

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