GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE52125 Query DataSets for GSE52125
Status Public on Mar 10, 2015
Title m6A RNA Methylation Is Regulated by MicroRNAs and Promotes Reprogramming to Pluripotency
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary N6-methyladenosine (m6A) has been recently identified as a conserved epitranscriptomic modification of eukaryotic mRNAs, but its features, regulatory mechanisms, and functions in cell reprogramming are largely unknown. Here, we report m6A modification profiles in the mRNA transcriptomes of four cell types with different degrees of pluripotency. Comparative analysis reveals several features of m6A, especially gene- and cell-type-specific m6A mRNA modifications. We also show that microRNAs (miRNAs) regulate m6A modification via a sequence pairing mechanism. Manipulation of miRNA expression or sequences alters m6A modification levels through modulating the binding of METTL3 methyltransferase to mRNAs containing miRNA targeting sites. Increased m6A abundance promotes the reprogramming of mouse embryonic fibroblasts (MEFs) to pluripotent stem cells; conversely, reduced m6A levels impede reprogramming. Our results therefore uncover a role for miRNAs in regulating m6A formation of mRNAs and provide a foundation for future functional studies of m6A modification in cell reprogramming.
Overall design m6A-seq in ESC, iPSC, NSC and sertoli cells.
Contributor(s) Chen T, Hao Y, Zhang Y, Wang X, Yang Y, Zhou Q
Citation(s) 25683224
Submission date Nov 06, 2013
Last update date May 15, 2019
Contact name Tong Chen
Phone 86-15210822685
Organization name China Academy of Chinese Medical Sciences
Department State Key Laboratory Breeding Base of Dao-di Herbs, National Resource Center for Chinese Materia Medica
Lab Luqi Huang's lab
Street address No 16 Nan-xiao-jie Dong-zhi-men-nei
City Beijing
ZIP/Postal code 100700
Country China
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (8)
GSM1260055 ESC_m6A-Seq
GSM1260056 iPSC_m6A-Seq
GSM1260057 NSC_m6A-Seq
BioProject PRJNA226754
SRA SRP032669

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE52125_Processed_data.chen.ct586.expr_modification_for_transcript.xlsx.gz 1.7 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap