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Series GSE52113 Query DataSets for GSE52113
Status Public on Sep 25, 2014
Title H3K4me1 marks DNA regions hypomethylated during aging in human stem and differentiated cells [twins]
Organism Homo sapiens
Experiment type Methylation profiling by array
Summary In differentiated cells, aging is associated with hypermethylation of DNA regions enriched in repressive histone posttranslational modifications. However, the chromatin marks associated with changes in DNA methylation in adult stem cells during lifetime are still largely unknown. Here, DNA methylation profiling of mesenchymal stem cells obtained from individuals aged 2 to 92 identified 18735 hypermethylated and 45407 hypomethylated CpG sites associated with aging. As in differentiated cells, hypermethylated sequences were enriched in chromatin repressive marks. Most importantly, hypomethylated CpG sites were strongly enriched in the active chromatin mark H3K4me1 in stem and differentiated cells, suggesting this is a cell type-independent chromatin signature of DNA hypomethylation during aging. Analysis of scedasticity showed that interindividual variability of DNA methylation increased during aging in MSCs and differentiated cells, providing a new avenue for the identification of DNA methylation changes over time. DNA methylation profiling of genetically identical individuals showed that both the tendency of DNA methylation changes and scedasticity depended on non-genetic as well as genetic factors. Our results indicate that the dynamics of DNA methylation during aging depend on a complex mixture of factors that include the DNA sequence, cell type and chromatin context involved, and that, depending on the locus, the changes can be modulated by genetic and/or external factors.
Overall design Total DNA isolated by standard procedures from peripheral blood of 24 samples obtained from 12 pairs of MZ twins aged 21 to 66.
Contributor(s) Fernandez AF, Bayón GF, Urdinguio RG, Toraño EG, Garcia MG, Carella A, Petrus-Reurer S, Ferrero C, Martinez-Camblor P, Cubillo I, García-Castro J, Delgado-Calle J, Perez-Campo FM, Riancho JA, Bueno C, Menendez P, Mentink A, Mareschi K, Claire F, Fagnani C, Medda E, Toccaceli V, Brescianini S, Moran S, Esteller M, Stolzing A, de Boer J, Nisticò L, Stazi MA, Fraga MF
Citation(s) 25271306
Submission date Nov 05, 2013
Last update date Mar 22, 2019
Contact name Agustin F Fernandez
Phone 985652411
Organization name Oviedo University-HUCA
Department IUOPA
Lab Epigenetics
Street address Avenida de Roma s/n
City Oviedo
State/province Asturias
ZIP/Postal code 33011
Country Spain
Platforms (1)
GPL13534 Illumina HumanMethylation450 BeadChip (HumanMethylation450_15017482)
Samples (24)
GSM1259870 Whole blood twin 7A
GSM1259871 Whole blood twin 7B
GSM1259872 Whole blood twin 11A
This SubSeries is part of SuperSeries:
GSE52114 H3K4me1 marks DNA regions hypomethylated during aging in human stem and differentiated cells
BioProject PRJNA226710

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE52113_RAW.tar 183.1 Mb (http)(custom) TAR
GSE52113_methylated_unmethylated_signals.txt.gz 72.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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