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| Status |
Public on Nov 05, 2013 |
| Title |
5'RNA-seq analysis of hypertrophic cardiomyopathy (HCM) in mice that carry a human missence mutation in the myosin heavy chain |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We developed a 5'RNA-seq methodology to concurrently assess gene expression and start-site usage changes. We applied this methodology to study hypertrophic cardiomyopathy in mice harboring a human deleterious mutation.
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| Overall design |
5'RNA-seq analysis of transcriptomes from mouse hearts with or without hypertrophic cardiomyopathy. Biological replicates were pooled into a single sequencing run. 5'RNA-seq methodology consists of enhanced sequencing of 5' ends and computational assessment of changes at start-sites of genes.
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| Contributor(s) |
Seidman J |
| Citation(s) |
24509080 |
| Submission date |
Nov 04, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Jonathan Seidman |
| Organization name |
Harvard Medical School
|
| Department |
Genetics
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| Lab |
Seidman
|
| Street address |
77 Avenue Louis Pasteur, NRB256
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA226563 |
| SRA |
SRP032512 |
