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| Status |
Public on Oct 30, 2013 |
| Title |
Association of CREB3L1 with promoters in LN4D6 CREB3L1 cells |
| Organism |
Rattus norvegicus |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
The unfolded protein response (UPR) is activated in response to hypoxia-induced stress such as in the tumor microenvironment. This study examined the role of CREB3L1 (cAMP-responsive element-binding protein 3-like protein 1), a member of the UPR, in breast cancer development and metastasis. Initial experiments identified the loss of CREB3L1 expression in metastatic breast cancer cell lines compared to low- or non-metastatic cell lines. When metastatic cells were transfected with CREB3L1 they demonstrated reduced invasion and migration in vitro, as well as a significantly decreased ability to survive under non-adherent or hypoxic conditions. Interestingly, in an in vivo rat mammary tumor model, CREB3L1 expressing cells not only failed to form metastases compared to CREB3L1 null cells but regression of the primary tumors was seen in 70% of the animals as a result of impaired angiogenesis. Microarray and ChIP on Chip analyses identified changes in the expression of many genes involved in cancer development and metastasis, including a decrease in those involved in angiogenesis. These data suggest that CREB3L1 plays an important role in suppressing tumorgenesis and loss of expression is required for the development of a metastatic phenotype.
CREB3L1 is a member of the unfolded protein response family of proteins. CREB3L1 expression is lost from metastatic breast cancer cells. We wanted to determine the promoters of genes that CREB3L1 bound to.
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| Overall design |
Idenification of promoters to which CREB3L1 is bound following ChIP with a HA antibody or control.
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| Contributor(s) |
Anderson D, Mellor P |
| Citation(s) |
24126059 |
| |
| Submission date |
Oct 28, 2013 |
| Last update date |
Jan 08, 2014 |
| Contact name |
Deborah Anderson |
| E-mail(s) |
deborah.anderson@saskcancer.ca
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| Phone |
13069667038
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| Organization name |
University of Saskatchewan
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| Street address |
107 Wiggins Road
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| City |
Saskatoon |
| State/province |
Saskatchewan |
| ZIP/Postal code |
S7N 5E5 |
| Country |
Canada |
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| Platforms (1) |
| GPL17848 |
NimbleGen Rat Promoter Array [2006-10-31_RN34_promoter_1of2] |
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| Samples (1) |
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| This SubSeries is part of SuperSeries: |
| GSE51857 |
CREB3L1 is a metastasis suppressor that represses expression of genes regulating metastasis, invasion and angiogenesis |
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| Relations |
| BioProject |
PRJNA225612 |
