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Series GSE51682 Query DataSets for GSE51682
Status Public on Sep 10, 2015
Title Zscan4 mediates transient remodeling and transcriptional burst of heterochromatin in mouse embryonic stem cells
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Expression profiling by high throughput sequencing
Summary This SuperSeries is composed of the SubSeries listed below.

Repetitive sequences such as telomeres, centromeres, and retrotransposons are packed in permanently-condensed and transcriptionally-silenced heterochromatin, whose maintenance is critical for the genome stability. We have recently found that mouse ES cells occasionally go through a unique cell state marked by the transient expression of Zscan4, which plays a key role in long-term genome stability. Here we report our unexpected and counterintuitive finding that the Zscan4+ state is accompanied by a Zscan4-mediated rapid activation and repression of heterochromatin: the burst of transcription from heterochromatin coincided with its transient histone hyperacetylation and DNA demethylation, the clustering of pericentromeric heterochromatin in the perinucleolar region, and the accumulation of chromatin remodeling complexes for both activation (HATs, SWI/SNF) and repression (HDACs, LSD1/KDM1A, NuRD) on heterochromatin. Overall, these findings suggest that ES cells maintain their extraordinary genome stability and immortality by transiently taking extreme measures – a usually forbidden transition to the activated state of heterochromatin.
Overall design Refer to individual Series
Citation(s) 26324425
Submission date Oct 24, 2013
Last update date May 15, 2019
Contact name Minoru S.H. Ko
Phone 410-558-8359
Organization name NIH
Department National Institute on Aging
Lab Lab of Genetics
Street address 251 Bayview Blvd, Suite 100, 10C
City Baltimore
State/province MD
ZIP/Postal code 21224
Country USA
Platforms (2)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (16)
GSM1250433 MC1-ZE7 cells (Em-) H3K27ac rep1
GSM1250434 MC1-ZE7 cells (Em-) H3K27ac rep2
GSM1250435 MC1-ZE7 cells (Em+ high fraction) H3K27ac rep1
This SuperSeries is composed of the following SubSeries:
GSE51679 ChIP-seq assays for H3K27ac
GSE51680 Genome-wide DNA methylation analyses by the HELP assay
GSE58617 ChIP-seq assays for H3K9me2
BioProject PRJNA224553

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE51682_RAW.tar 49.2 Mb (http)(custom) TAR (of TXT)
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