|
| Status |
Public on Sep 10, 2015 |
| Title |
ChIP-seq assays for H3K27ac |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
By using FACS-sorted MC1-ZE7 ES cells, we analyzed the genome-wide distribution of H3K27ac and DNA methylation in Zscan4 positive ES cells (Em+) and Zscan4 negative ES cells (Em-). H3K27 hyperacetylation and DNA demethylation were detected in heterochromatic regions of Em+ cells, but not Em- cells. These results suggested that the Zscan4 state takes "open" chromatin conformation in ES cells.
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| |
|
| Overall design |
H3K27ac in Zscan4 positive and negative cells
|
| |
|
| Contributor(s) |
Akiyama T |
| Citation(s) |
26324425 |
| Submission date |
Oct 24, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Minoru S.H. Ko |
| E-mail(s) |
kom@mail.nih.gov
|
| Phone |
410-558-8359
|
| Organization name |
NIH
|
| Department |
National Institute on Aging
|
| Lab |
Lab of Genetics
|
| Street address |
251 Bayview Blvd, Suite 100, 10C
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21224 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
|
| Samples (4)
|
| GSM1250436 |
MC1-ZE7 cells (Em+ high fraction) H3K27ac rep2 |
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| This SubSeries is part of SuperSeries: |
| GSE51682 |
Zscan4 mediates transient remodeling and transcriptional burst of heterochromatin in mouse embryonic stem cells |
|
| Relations |
| BioProject |
PRJNA224548 |
| SRA |
SRP031895 |
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