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Series GSE51627 Query DataSets for GSE51627
Status Public on Oct 24, 2013
Title Treatment of renal cell carcinoma cell line A-498 with zebularine
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Cultures of A-498 cells were treated for 120hours with 1000µM zebularine (SIGMA) in three independent biological experiments. Zebularine acts as a DNA methyltransferase (DNMT) inhibitor thereby upregulating genes that are inactivated by e.g. promotor hypermethylation. The experiment aimed to search for upregulated transcripts to provide new targets for biomarker development and therapeutic use. 308 candidates were upregulated more than 1.5-fold. Members of the metallothionein group (MT1G, MT1H, and MT2A) were validated in 49 clinical samples of renal cell carcinomas.
Overall design Total RNA of treated (1000µM zebularine) and untreated A-498 cells (experiment A05-A07) was subjected to Affymetrix array analysis to detail the overall expression changes after treatment with a DNMT inhibitor. Treated cells showed no obvious signs of zebularine-induced cytotoxicity as revealed by XTT assays. Cell were split twice during the 120hour treatment period.
Contributor(s) Krause HG, Al-Kamal I
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Submission date Oct 23, 2013
Last update date Dec 06, 2018
Contact name Hans G Krause
Organization name Charité - Universitätsmedizin Berlin
Department Klinik für Urologie
Street address Charitéplatz 1
City Berlin
ZIP/Postal code 10117
Country Germany
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (6)
GSM1249683 Untreated control, A-498, biological rep 1
GSM1249684 1000µM zebularine, A-498, biological rep 1
GSM1249685 Untreated control, A-498, biological rep 2
BioProject PRJNA224109

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Supplementary file Size Download File type/resource
GSE51627_RAW.tar 11.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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