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Series GSE5144 Query DataSets for GSE5144
Status Public on Jun 24, 2006
Title hTERT effects in CD8 T Lymphocytes from two healthy donors
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Expression in GFP vs. GFP/hTERT transduced CD8 T Lymphocytes from Healty Donors (HD) 1 and 2 at early and late passages. Using CD8+ T lymphocyte clones over-expressing telomerase we investigated the molecular mechanisms that regulate T cell proliferation. Transduction and subcloning procedures were performed on CD8 + naive T-cell clones isolated from two different healthy individuals aged between 30 to 35 years (HD1 and HD2). T-cell cloneswere transduced to express hTERT/GFP or GFP alone.
Keywords: Cell Line Comparison.
 
Overall design triplicates of 4 conditions for each of 2 donors, 1 chip (hTERT_YOUNG_HD1_REP2) of the first donor excluded because of low quality hybridization, one condition (GFP YOUNG)lost from second donor because of problems in cell culture.
 
Contributor(s) Rufer N, Menzel O, Migliaccio M, Goldstein D, Dahoun S, Delorenzi M
Citation(s) 16951325
Submission date Jun 23, 2006
Last update date Mar 25, 2019
Contact name Mauro Delorenzi
E-mail(s) Mauro.Delorenzi@unil.ch
Organization name Swiss Institute of Bioinformatics
Lab BCF
Street address GĂ©nopode Building, Quartier Sorge
City Lausanne
ZIP/Postal code 1015
Country Switzerland
 
Platforms (2)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (20)
GSM116081 GFP_YOUNG_HD1_REP1
GSM116082 GFP_YOUNG_HD1_REP2
GSM116083 GFP_YOUNG_HD1_REP3
Relations
BioProject PRJNA96659

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