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Series GSE51242 Query DataSets for GSE51242
Status Public on Dec 31, 2016
Title A Molecular Profile of Colorectal Cancer to Guide Therapy [HMEC]
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Here we describe the generation and application of a gene expression signature for the Rheb pathway. Affymetrix array data obtained from human mammary epithelial cells over expressing Rheb or GFP were used to generate a Rheb/mTOR activation signature. To test the capacity of the signature to predict patient response to everolimus we treated patient-derived colorectal cancer explants (PDCCEs) predicted to have high and low Rheb pathway activity withthe mTOR inhibitor everolimus.
Overall design This study utilized a total of 20 affymetrix data files generated from 20 independent human mammary epithelial cell culture samples treated with adenovirus overexpressing either Rheb (n=10) or GFP (n=10). From these data, a Rheb gene expression signature was created and used to predict Rheb/mTOR activity in patient derived colorectal cancer explants (PDCCEs). PDCCEs were then tested for response to the mTOR inhibitor everolimus.
Contributor(s) Uronis J, VanDeusen J, Proussaloglou E, Osada T, Yang XY, Mantyh C, Morse MA, Lyerly HK, Clary BM, Gatza M, Datto M, Chang J, Hsu DS
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Submission date Sep 27, 2013
Last update date Dec 06, 2018
Contact name David Hsu
Organization name Duke University
Department Medical Oncology
Street address 905 S. LaSalle St. Rm 3008
City Durham
State/province NC
ZIP/Postal code 27710
Country USA
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (20)
GSM1240932 Human Mammary Epithelial Cells_Rheb_transfected_1
GSM1240933 Human Mammary Epithelial Cells_Rheb_transfected_2
GSM1240934 Human Mammary Epithelial Cells_Rheb_transfected_3
This SubSeries is part of SuperSeries:
GSE51244 A Molecular Profile of Colorectal Cancer to Guide Therapy
BioProject PRJNA221801

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE51242_RAW.tar 38.5 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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