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| Status |
Public on Dec 31, 2016 |
| Title |
A Molecular Profile of Colorectal Cancer to Guide Therapy [HMEC] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Here we describe the generation and application of a gene expression signature for the Rheb pathway. Affymetrix array data obtained from human mammary epithelial cells over expressing Rheb or GFP were used to generate a Rheb/mTOR activation signature. To test the capacity of the signature to predict patient response to everolimus we treated patient-derived colorectal cancer explants (PDCCEs) predicted to have high and low Rheb pathway activity withthe mTOR inhibitor everolimus.
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| Overall design |
This study utilized a total of 20 affymetrix data files generated from 20 independent human mammary epithelial cell culture samples treated with adenovirus overexpressing either Rheb (n=10) or GFP (n=10). From these data, a Rheb gene expression signature was created and used to predict Rheb/mTOR activity in patient derived colorectal cancer explants (PDCCEs). PDCCEs were then tested for response to the mTOR inhibitor everolimus.
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| Contributor(s) |
Uronis J, VanDeusen J, Proussaloglou E, Osada T, Yang XY, Mantyh C, Morse MA, Lyerly HK, Clary BM, Gatza M, Datto M, Chang J, Hsu DS |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 27, 2013 |
| Last update date |
Dec 06, 2018 |
| Contact name |
David Hsu |
| Organization name |
Duke University
|
| Department |
Medical Oncology
|
| Street address |
905 S. LaSalle St. Rm 3008
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27710 |
| Country |
USA |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (20)
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| GSM1240932 |
Human Mammary Epithelial Cells_Rheb_transfected_1 |
| GSM1240933 |
Human Mammary Epithelial Cells_Rheb_transfected_2 |
| GSM1240934 |
Human Mammary Epithelial Cells_Rheb_transfected_3 |
| GSM1240935 |
Human Mammary Epithelial Cells_Rheb_transfected_4 |
| GSM1240936 |
Human Mammary Epithelial Cells_Rheb_transfected_5 |
| GSM1240937 |
Human Mammary Epithelial Cells_Rheb_transfected_6 |
| GSM1240938 |
Human Mammary Epithelial Cells_Rheb_transfected_7 |
| GSM1240939 |
Human Mammary Epithelial Cells_Rheb_transfected_8 |
| GSM1240940 |
Human Mammary Epithelial Cells_Rheb_transfected_9 |
| GSM1240941 |
Human Mammary Epithelial Cells_Rheb_transfected_10 |
| GSM1240942 |
Human Mammary Epithelial Cells_GFP_Transfected_1 |
| GSM1240943 |
Human Mammary Epithelial Cells_GFP_Transfected_2 |
| GSM1240944 |
Human Mammary Epithelial Cells_GFP_Transfected_3 |
| GSM1240945 |
Human Mammary Epithelial Cells_GFP_Transfected_4 |
| GSM1240946 |
Human Mammary Epithelial Cells_GFP_Transfected_5 |
| GSM1240947 |
Human Mammary Epithelial Cells_GFP_Transfected_6 |
| GSM1240948 |
Human Mammary Epithelial Cells_GFP_Transfected_7 |
| GSM1240949 |
Human Mammary Epithelial Cells_GFP_Transfected_8 |
| GSM1240950 |
Human Mammary Epithelial Cells_GFP_Transfected_9 |
| GSM1240951 |
Human Mammary Epithelial Cells_GFP_Transfected_10 |
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| This SubSeries is part of SuperSeries: |
| GSE51244 |
A Molecular Profile of Colorectal Cancer to Guide Therapy |
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| Relations |
| BioProject |
PRJNA221801 |
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