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Status |
Public on Jan 21, 2014 |
Title |
Androgen receptor uses relaxed response element stringency for selective chromatin binding and transcriptional regulation in vivo |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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Summary |
We report the in vivo androgen receptor (AR) binding sites in caput epididymis of intact SPARKI and wild-type mice using ChIP-sequencing. SPARKI (specificity affecting androgen receptor knock-in) mouse line has the second zinc finger of AR replaced by that of glucocorticoid receptors. In vivo analysis of SPARKI and wild-type AR genome-wide binding sites identified cis-element with less stringent sequence requirements that specify selective AR-binding sites. The ChIP experiments have been performed using antibody specific to AR and IgG non-specific antibody as a negative control.
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Overall design |
Examination of AR binding sites in epididymis of wild-type and SPARKI mice using ChIP-seq. Two biological replicates from intact wild-type and SPARKI mice and two control IgG samples were sequenced and used in peak calling. To increase the depth of the analysis, replicate ChIP-seq samples were merged and the concatanated samples were used in peak calling.
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Contributor(s) |
Sahu B, Pihlajamaa P, Dubois V, Kerkhofs S, Claessens F, Jänne OA |
Citation(s) |
24459135 |
Submission date |
Sep 23, 2013 |
Last update date |
May 15, 2019 |
Contact name |
Olli A. Jänne |
E-mail(s) |
olli.janne@helsinki.fi
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Phone |
+358919125040
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Organization name |
University of Helsinki
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Department |
Inst. of Biomedicine/Physiology
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Lab |
Androgen Receptor Laboratory
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Street address |
Haartmaninkatu-8
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City |
Helsinki |
ZIP/Postal code |
FI-00014 |
Country |
Finland |
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Platforms (1) |
GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
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Samples (9)
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Relations |
BioProject |
PRJNA221193 |
SRA |
SRP030212 |