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Series GSE50958 Query DataSets for GSE50958
Status Public on Sep 17, 2016
Title Genome-wide epigenetic analyses and Transcriptional analysis in NB4 cells expressing PML-RARalpha
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Illumina High-Throughput ChIP Sequencing profiling was performed using the H3K9K14ac antibody in NB4 cells treated with the compounds ATRA, MS-275,MC2392 (a hybrid molecule of ATRA with a 2-aminoanilide tail of the HDAC inhibitor MS-275) or solvent, DMSO. We find that MC2392 induces changes in H3 acetylation at a small subset of PML-RARα binding sites but also in regions not regulated by ATRA. Moreover, MC2392 alters expression of a number of stress-responsive and apoptotic genes.
Overall design Genome-wide ChIP-seq was performed in NB4 cells for histone3 acetylated in K9K14
Contributor(s) De Bellis F, Carafa V, Nebbioso A, Rotili D, Petraglia F, Conte M, Francoijs K, Ablain J, Valente S, Castellano R, Goubard A, Collette Y, Matarese F, Martens J, de Thé H, Mai A, Stunnenberg HG, Altucci L
Citation(s) 24566867
Submission date Sep 17, 2013
Last update date May 15, 2019
Contact name H.G. Stunnenberg
Phone +31-24-3610520
Organization name Radboud University
Department Department of Molecular Biology (274)
Street address Geert Grooteplein 28
City Nijmegen
ZIP/Postal code 6525 GA
Country USA
Platforms (2)
GPL9052 Illumina Genome Analyzer (Homo sapiens)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
Samples (12)
GSM1233292 NB4_DMSO_ChIPSeq
GSM1233293 NB4_ATRA_4h_ChIPSeq
GSM1233294 NB4_ATRA_24h_ChIPSeq
BioProject PRJNA219427
SRA SRP029992

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Supplementary file Size Download File type/resource
GSE50958_RAW.tar 1.5 Gb (http)(custom) TAR (of BED, WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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