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Series GSE50900 Query DataSets for GSE50900
Status Public on Nov 25, 2016
Title Gene profiling of the zone of altered morphology of choriodecidua overlying the cervix before labor
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To characterize the molecular signature of the choriodecidua remodeling in late pregnancy befour labour, we have employed whole genome microarray expression profiling to identify genes that sign for fetal membranes remodeling. Fetal membranes were collected from healthy women, after an uncomplicated pregnancy, undergoing a planned cesarean delivery at term, for feto-pelvic disproportion or scarred uterus. During surgery, a Kocher's forceps was placed on membranes overlying the cervix and the swelling area surrounding the forceps was dissected in the operating room under sterile conditions, the site, pinched by the forceps, being discarded. Fetal membranes in a remote zone were also sampled. Isolated RNAs were hybridized to the Aligent SurePrint G3 Human GE 8x60K Microarray. Two independent bio-analyses singularize a signature in the zone of altered morphology for Graft vs Host Disease and leukocyte activation, arguing for a focal loss of maternal-fetal tolerance. This was further confirmed by flow cytometry, electron microscopy, and immuhistochemistry analyses.
Overall design Microarray analysis was conducted on three distinct groups of three paired sets of zone of altered morphology and intact morphology from nine pregnant women in total. These women were delivered at term by cesarean sectionfor feto-pelvic disproportion or scarred uterus, after an uncomplicated pregnancy.
Contributor(s) Méhats C, Marcellin L, Jacques S
Citation(s) 28031337
Submission date Sep 16, 2013
Last update date Nov 27, 2018
Contact name Céline Méhats
Organization name Inserm
Department U1016
Street address 24 rue du Faubourg St jacques
City Paris
ZIP/Postal code 75014
Country France
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (6)
GSM1232168 ZIM_replicate1
GSM1232169 ZIM_replicate2
GSM1232170 ZIM_replicate3
BioProject PRJNA219318

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Supplementary file Size Download File type/resource
GSE50900_RAW.tar 26.2 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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