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Series GSE50838 Query DataSets for GSE50838
Status Public on Sep 09, 2014
Title Expression data from activated NK cells [RNA]
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Natural Killer (NK) cells present natural cytotoxicity against tumor cells, although their activity is increased after activation. NK cell activation depends on a complex intracellular signaling process mediated by activating and inhibitory receptors and the functional outcome depends on the integration of the activating and inhibitory signals received. Soluble cytokines and/or ligands on target cells bind the NK cell receptors, and hence, influence the final NK cell response: attack versus ignorance.
We used microarrays to detail the global programme of gene expression underlying NK cell activation by IL-2, a MHC-I-deficient target cell (K562)+IL-2 and an EBV-target cell (R69).
Overall design PBLs from 4 different donors were activated by 100 U/ml IL-2; K562+IL-2 or R69 cells. After 5 days we obtained RNA and miRNA from naïve NK cells or from NK cells activated with the above mentioned stimuli, with more than 90% of purity. The 16 RNA samples were used to generate cDNA libraries that were hybridized on Human Gene 1.1ST arrays (Affymetrix) and analyzed with the Affymetrix Gene Chip Command Console Software v3.0 (AGCC 3.0, Affymetrix®) and the Expression Console Software v1.1 (Affymetrix®).
Contributor(s) Villalba M, Pardo J
Citation(s) 24440757
Submission date Sep 12, 2013
Last update date Nov 08, 2016
Contact name martin villalba
Phone 33467330465
Organization name Institut for Research in Biotherapy of Montpellier
Department INSERM U1040
Street address CHU Montpellier Hôpital Saint-Eloi 80, av. Augustin Fliche.
City montpellier
ZIP/Postal code 34295
Country France
Platforms (1)
GPL11532 [HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version]
Samples (16)
GSM1231002 1-naive
GSM1231003 2-naive
GSM1231004 3-naive
This SubSeries is part of SuperSeries:
GSE50840 Expression data from activated NK cells
BioProject PRJNA219135

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Supplementary file Size Download File type/resource
GSE50838_RAW.tar 72.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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