GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE50809 Query DataSets for GSE50809
Status Public on Aug 25, 2014
Title Temporally defined neocortical translation and polysome assembly is determined by the RNA-binding protein, Hu antigen R
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Precise spatiotemporal control of mRNA translation machinery is essential to proper development of highly complex systems like the neocortex. Here, we show that an RNA-binding protein, Hu antigen R (HuR), regulates both neocorticogenesis and specificity of neocortical translation machinery in a developmental stage-dependent manner in mice. Neocortical absence of HuR alters the phosphorylation states of the initiation and elongation factors of the core translation machinery. In addition, HuR regulates the temporally specific positioning of functionally related mRNAs into the active translation sites, the polysomes. HuR also determines the specificity of neocortical polysomes by defining their combinatorial composition of ribosomal proteins and initiation and elongation factors. For some of the HuR-dependent proteins, the association with polysomes depends on the eIF2 alpha kinase 4 (eIF2ak4), which associated with HuR in prenatal developing neocortices. Finally, we found that deletion of HuR prior to embryonic day 10 (E10) disrupts both neocortical lamination and formation of the main neocortical commissure, the corpus callosum. Our study identifies a crucial role for HuR in neocortical development as a translational gatekeeper for functionally related mRNA subgroups and polysomal protein specificity.
Overall design Cortex was dissected from WT and HuR cKO mouse pups at embryonic day 13 (E13) or the day of birth (P0).
Contributor(s) Hart RP, Rasin MR, Kraushar ML, Thompson K
Citation(s) 25157170, 26245956
Submission date Sep 12, 2013
Last update date May 15, 2019
Contact name Ronald P. Hart
Phone 848-445-1783
Organization name Rutgers University
Department Cell Biology & Neuroscience
Street address 604 Allison Rd Rm B430
City Piscataway
State/province NJ
ZIP/Postal code 08854
Country USA
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (31)
GSM1229976 M_E13_WT_0
GSM1229977 M_E13_WT_1
GSM1229978 M_P0_WT_0
BioProject PRJNA219065
SRA SRP029912

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE50809_fpkm-replicates-WT.txt.gz 1009.4 Kb (ftp)(http) TXT
GSE50809_fpkm-replicates-cKO.txt.gz 1.0 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap