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Series GSE50806 Query DataSets for GSE50806
Status Public on Sep 13, 2013
Title Specification of innate type-2 lymphocytes by the transcriptional determinant Gfi1
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Innate type-2 lymphoid cells (ILC2s) function in immune responses against helminth parasites and are implicated in allergic inflammation and asthma. ILC2s are activated by the epithelial-derived cytokines IL-33 and IL-25 and are major sources of the type-2 cytokines IL-5 and IL-13. We show that the transcription factor Gfi1 promotes the generation of ILC2s and controls their responsiveness during Nippostrongylus brasiliensis infection as well as IL-33- or IL-25-instigated inflammation. Gfi1 directly activates Il1rl1, which encodes the IL-33 receptor. IL- 33 signaling upregulates Gfi1, thereby constituting a positive feedback loop that enables rapid and robust expansion of ILC2s in response to IL-33 signaling. Loss of Gfi1 in activated ILC2s results in an unusual effector state involving derepression of the IL-17 inflammatory program and co-expression of IL-13 with IL-17. ChIPseq reveals key Gfi1 targeted genes that are activated or repressed to maintain ILC2 identity. We propose that Gfi1 functions as a shared determinant within innate and adaptive immune cells to specify type-2 responses, while actively repressing the IL-17 effector state.
Overall design ILC2s (~3 x 10^7 cells) were sorted from the MLN of IL-25-treated mice. Chromatin fragments bound by Gfi1 were subject to ChIP using Gfi1 antibodies and followed by high-throughput sequencing.
Contributor(s) Spooner CJ, Khan AA, Singh H
Citation(s) 24141388
Submission date Sep 12, 2013
Last update date May 15, 2019
Contact name Aly Azeem Khan
Organization name University of Chicago
Street address 6045 S. Kenwood Ave.
City Chicago
State/province IL
ZIP/Postal code 60637
Country USA
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (2)
GSM1229967 ILC2_Gfi1_ChIP
GSM1229968 input DNA
BioProject PRJNA219059
SRA SRP029908

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Supplementary file Size Download File type/resource
GSE50806_gfi1_peaks.csv.gz 91.2 Kb (ftp)(http) CSV
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Processed data are available on Series record
Raw data are available in SRA

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